Species Reactivity : Human (Homo sapiens)
UniProt : P49281
Abbreviation : SLC11A2
Alternative Names : DCT1; DMT1; FLJ37416; NRAMP2; natural resistance-associated macrophage protein 2
Application : ELISA
Range : 0.156-10 ng/mL
Sensitivity : 0.055 ng/mL
Intra-AssayCV : ?4.6%
Inter-AssayCV : ?6.6%
Recovery : 0.95
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate SLC11A2 in samples. An antibody specific for SLC11A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC11A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for SLC11A2 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC11A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : The SLC11A2 gene encodes a divalent metal transporter (DMT1), which carries iron, manganese, cobalt, nickel, cadmium, lead, copper, and zinc. DMT1 participates in cell µLar iron absorption at the luminal surface of the duodenum as well as in other areas of the body. Predicted amino acid sequence analysis of the NRAMP2 polypeptide identified a polytopic membrane protein highly homologous to human NRAMP1 with 66% identical residues (80% overall homology), res µLting in identical predicted secondary structure of the 2 proteins. Sequence conservation is partic µLarly high in the predicted transmembrane domains (90%), s µggesting that these regions play a key role in the structural and functional aspects common to both proteins. As opposed to its NRAMP1 counterpart, whose expression is restricted to phagocytic cells.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).