Species Reactivity : Human (Homo sapiens)
UniProt : Q8N608
Abbreviation : DPP10
Alternative Names : DPL2; DPPY; DPRP3; OTTHUMP00000203618|dipeptidyl peptidase 10|dipeptidyl peptidase IV-related protein 3|dipeptidyl peptidase X|dipeptidyl peptidase like protein 2
Application : ELISA
Range : 0.312-20 ng/mL
Sensitivity : 0.116 ng/mL
Intra-AssayCV : ?5.8%
Inter-AssayCV : ?7.7%
Recovery : 1.06
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate DPP10 in samples. An antibody specific for DPP10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDPP10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for DPP10 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPP10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : DPP10 encodes a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. This protein has no detectable protease activity, most likely due to the absence of the conserved serine residue normally present in the catalytic domain of serine proteases. However, it does bind specific voltage-gated potassium channels and alters their expression and biophysical properties. Mutations in this gene have been associated with asthma. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. DPP10 lacks the active-site serine, which is substituted with a glycine residue. Database analysis s µggested the presence of a second DPP10 transcript. DPP10 shares 48% and 51% amino acid identity with the short and long DPP6 isoforms, respectively.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).