Species Reactivity : Human (Homo sapiens)
UniProt : P01730
Abbreviation : CD4
Alternative Names : CD4mut; CD4 antigen|CD4 antigen (p55)|CD4 receptor
Application : ELISA
Range : 0.156-10 ng/mL
Sensitivity : 0.064 ng/mL
Intra-AssayCV : ?3.5%
Inter-AssayCV : ?6.7%
Recovery : 1.06
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate CD4 in samples. An antibody specific for CD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCD4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for CD4 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, reg µLatory T cells, monocytes, macrophages, and dendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 (after the OKT4 monoclonal antibody that reacted with it) before being named CD4 in 1984.In humans, the CD4 protein is encoded by the CD4 gene. CD4 is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR by recruiting an enzyme, known as the tyrosine kinase lck, which is essential for activating many molec µLes involved in the signaling cascade of an activated T cell.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).