Species Reactivity : Human (Homo sapiens)
UniProt : N/A
Abbreviation : ADRA1-Ab-IgG
Alternative Names : N/A
Application : ELISA
Range : 0.312-20 ng/mL
Sensitivity : 0.119 ng/mL
Intra-AssayCV : ?5.9%
Inter-AssayCV : ?8.4%
Recovery : 1.05
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Qualitative
Test principle : This assay employs a two-site sandwich ELISA to quantitate ADRA1-Ab-IgG in samples. An antibody specific for ADRA1-Ab-IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADRA1-Ab-IgG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for ADRA1-Ab-IgG is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADRA1-Ab-IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : The alpha 1c- Adrenergic Receptor, also known as alpha 1a-adrenoceptor, is activated by epinephrine and norepinephrine. This receptor mediates calcium release upon ligand binding. The alpha 1-c adrenoceptor is one of three subtypes of alpha 1-adrenergic receptor (alpha 1 c/a, alpha 1b, and alpha 1d). The alpha 1c receptor couples to phospholipase C via a GTP-binding protein insensitive to pertussis toxin. Three isoforms are generated by alternative splicing. However, the corresponding isoforms of the protein have similar ligand-binding properties, effector coupling, and tissue distribution. Expression of this receptor has been reported in prostate, liver, kidney, heart, brain, spinal cord, bladder, and various blood vessels. ESTs have been isolated from liver and testis libraries.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).