Species Reactivity : Human (Homo sapiens)
UniProt : P25025
Abbreviation : IL8RB
Alternative Names : CD182; CDw128b; CMKAR2; IL8R2; IL8RA; IL8RB; CXCR2 gene for IL8 receptor type B|GRO/MGSA receptor|chemokine (CXC) receptor 2|high affinity interleukin-8 receptor B|interleukin 8 receptor B|interleuk
Application : ELISA
Range : 15.6-1000 pg/mL
Sensitivity : 6.2 pg/mL
Intra-AssayCV : ?5.7%
Inter-AssayCV : ?9.8%
Recovery : 1
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate IL8RB in samples. An antibody specific for IL8RB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL8RB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for IL8RB is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL8RB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Interleukin 8 receptor, beta is a chemokine receptor. IL8RB is also known as CXCR2, and CXCR2 is now the IUPHAR Committee on Receptor Nomenclature and Dr µg classification-recommended name.The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal thro µgh a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stim µLating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. In addition, it binds ligands CXCL2, CXCL3, and CXCL5.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).