Volume : 100 µg
Purification : Immunogen affinity purified
Form : liquid
Purity : 95% as determined by SDS-PAGE
Host : Rabbit
Clonality : polyclonal Ab
Clone ID :
Isotype : IgG
Storage : PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.)
Background :
Immunogen : major histocompatibility complex, class I, C
Aliases : D6S204, HLA C, HLA Cw, HLA Cw12, HLA JY3, HLAC, HLA-C, HLC C, MHC class I antigen Cw*7, PSORS1
Observed MW : 44 kDa
Uniprot ID : P30508
Reactivity : Human
Application : ELISA, WB, IHC, IF, IP
Recommended dilution : WB : 1 : 500-1 : 2000; IP : 1 : 500-1 : 2000; IHC : 1 : 20-1 : 200; IF : 1 : 50-1 : 500
Gene ID : 3107
Research Area : Cardiovasc µLar, Immunology, Cancer, Stem Cells, Cell Division and Proliferation, Signal Transduction

Volume : 100 µg
Purification : Immunogen affinity purified
Form : liquid
Purity : 95% as determined by SDS-PAGE
Host : Rabbit
Clonality : polyclonal Ab
Clone ID :
Isotype : IgG
Storage : PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.)
Background :
Immunogen : major histocompatibility complex, class I, B
Aliases : AS, HLA B, HLA B 7301 HLA B73, HLAB, HLA-B, HLAC, MHC class I antigen B*7, SPDA1
Observed MW : 45kd
Uniprot ID : P18464
Reactivity : Human
Application : ELISA, WB, IHC, IF, IP
Recommended dilution : WB : 1 : 100-1 : 4000; IP : 1 : 1000-1 : 4000; IHC : 1 : 20-1 : 200; IF : 1 : 20-1 : 200
Gene ID : 3106
Research Area : Cardiovasc µLar, Immunology, Cancer, Stem Cells, Cell Division and Proliferation, Signal Transduction

Volume : 100 µg
Purification : Immunogen affinity purified
Form : liquid
Purity : 95% as determined by SDS-PAGE
Host : Rabbit
Clonality : polyclonal Ab
Clone ID :
Isotype : IgG
Storage : PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.)
Background : HLA-A belongs to the HLA class I heavy chain paralogues. This class I molec µLe is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglob µLin). The heavy chain is anchored in the membrane. Class I molec µLes play a central role in the immune system by presenting peptides derived from the endoplasmic retic µLum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molec µLe. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.
Immunogen : major histocompatibility complex, class I, A
Aliases : HLA A, HLAA, HLA-A
Observed MW : 44 kDa
Uniprot ID : P04439/P30443
Reactivity : Human, Mouse
Application : ELISA, WB, IHC
Recommended dilution : WB : 1 : 500-1 : 2000; IHC : 1 : 50-1 : 200
Gene ID : 3105
Research Area : Cardiovasc µLar, Immunology, Cancer, Stem Cells, Cell Division and Proliferation, Signal Transduction

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : HLA-A
GeneID : 3105,
Uniprot : P04439
Specificity : HLA Class I Antibody detects endogenous levels of total HLA Class I.
Immunogen : A syntheVolumed peptide derived from human HLA Class I, corresponding to a region within C-terminal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse,Rat
Prediction :
Application : WB,ELISA(peptide)
Dilution : WB 1 : 500-1 : 2000, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2846408
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : DF13389-BP
Des# Blocking Peptide : HLA Class I Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : HKR1
GeneID : 284459
Uniprot : P10072
Specificity : HKR1 Antibody detects endogenous levels of total HKR1.
Immunogen : A syntheVolumed peptide derived from human HKR1 corresponding to a region within the internal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse
Prediction :
Application : WB,IHC,IF/ICC,ELISA(peptide)
Dilution : WB 1 : 500-1 : 1000, IF/ICC 1 : 100-1 : 500, IHC 1 : 50-1 : 200, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2835467
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : DF3084-BP
Des# Blocking Peptide : HKR1 Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 50 µg
Clone Number :
Aliases : BC016235 antibody; Hexokinase domain containing 1 antibody; Hexokinase domain-containing protein 1 antibody; Hkdc1 antibody; HKDC1_HUMAN antibody; Putative hexokinase HKDC1 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q2TB90
Immunogen : Recombinant Human Putative hexokinase HKDC1 protein (129-427AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background :
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HKDC1
Research Areas : Metabolism; Signal transduction

Volume : 50 µg
Clone Number :
Aliases : BC016235 antibody; Hexokinase domain containing 1 antibody; Hexokinase domain-containing protein 1 antibody; Hkdc1 antibody; HKDC1_HUMAN antibody; Putative hexokinase HKDC1 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q2TB90
Immunogen : Recombinant Human Putative hexokinase HKDC1 protein (129-427AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background :
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HKDC1
Research Areas : Metabolism; Signal transduction

Volume : 50 µg
Clone Number :
Aliases : BC016235 antibody; Hexokinase domain containing 1 antibody; Hexokinase domain-containing protein 1 antibody; Hkdc1 antibody; HKDC1_HUMAN antibody; Putative hexokinase HKDC1 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q2TB90
Immunogen : Recombinant Human Putative hexokinase HKDC1 protein (129-427AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background :
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Biotin
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HKDC1
Research Areas : Metabolism; Signal transduction

Volume : 50 µg
Clone Number :
Aliases : BC016235 antibody; Hexokinase domain containing 1 antibody; Hexokinase domain-containing protein 1 antibody; Hkdc1 antibody; HKDC1_HUMAN antibody; Putative hexokinase HKDC1 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q2TB90
Immunogen : Recombinant Human Putative hexokinase HKDC1 protein (129-427AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA, WB, IHC; Recommended dilution : WB : 1 : 1000-1 : 5000, IHC : 1 : 20-1 : 200
Background :
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Non-conj µgated
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HKDC1
Research Areas : Metabolism; Signal transduction

Volume : 100 µg
Purification : Immunogen affinity purified
Form : liquid
Purity : 95% as determined by SDS-PAGE
Host : Rabbit
Clonality : polyclonal Ab
Clone ID :
Isotype : IgG
Storage : PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20? for 12 months (Avoid repeated freeze / thaw cycles.)
Background : Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate.
Immunogen : hexokinase 3 (white cell)
Aliases : HXK3
Observed MW : 99 kDa
Uniprot ID : P52790
Reactivity : Human, Mouse, Rat
Application : ELISA, IHC, WB
Recommended dilution : WB : 1 : 500 - 1 : 2000; IHC : 1 : 50 - 1 : 100
Gene ID : 3101
Research Area : Signal Transduction, Metabolism

Volume : 100 µL
Clone Number :
Aliases : hexokinase-2 HK II hexokinase type II hexokinase-2, muscle muscle form hexokinase mi-2 autoantigen 240 kDa protein zinc-finger helicase (Snf2-like)
Product Type : Monoclonal Antibody
Immunogen Species : Human
UniProt ID : P52789
Immunogen : Recombinant fragment, corresponding to amino acids 270-435aa of human HK2(N-terminus).
Raised in : Mouse
Species Reactivity : Human,Pig,Mouse,Rat
Tested Applications : ELISA,WB,FC;Recommended dilution : WB : 1 : 500-1 : 5000
Background :
Clonality : Monoclonal
Isotype : IgG1
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HK2
Research Areas : Cancer?Cardiovasc µLar;Metabolism;Signal transduction

Volume : 50 µg
Clone Number :
Aliases : DKFZp686M1669 antibody; Hexokinase 2 antibody; Hexokinase 2 muscle antibody; Hexokinase type II antibody; Hexokinase-2 antibody; HK 2 antibody; HK II antibody; HK2 antibody; HKII antibody; HxK 2 antibody; HxK2 antibody; HXK2_HUMAN antibody; Muscle form hexokinase antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P52789
Immunogen : Recombinant Human Hexokinase-2 protein (334-526AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background :
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HK2
Research Areas : Cancer; Cardiovasc µLar; Metabolism; Signal transduction

Volume : 50 µg
Clone Number :
Aliases : DKFZp686M1669 antibody; Hexokinase 2 antibody; Hexokinase 2 muscle antibody; Hexokinase type II antibody; Hexokinase-2 antibody; HK 2 antibody; HK II antibody; HK2 antibody; HKII antibody; HxK 2 antibody; HxK2 antibody; HXK2_HUMAN antibody; Muscle form hexokinase antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P52789
Immunogen : Recombinant Human Hexokinase-2 protein (334-526AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background :
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HK2
Research Areas : Cancer; Cardiovasc µLar; Metabolism; Signal transduction

Volume : 50 µg
Clone Number :
Aliases : DKFZp686M1669 antibody; Hexokinase 2 antibody; Hexokinase 2 muscle antibody; Hexokinase type II antibody; Hexokinase-2 antibody; HK 2 antibody; HK II antibody; HK2 antibody; HKII antibody; HxK 2 antibody; HxK2 antibody; HXK2_HUMAN antibody; Muscle form hexokinase antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P52789
Immunogen : Recombinant Human Hexokinase-2 protein (334-526AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background :
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Biotin
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HK2
Research Areas : Cancer; Cardiovasc µLar; Metabolism; Signal transduction

Volume : 100 µL
Host : Mouse
Clonality : Monoclonal
Isotype : IgG1
GeneName : HK2
GeneID : 3099
Uniprot : P52789
Specificity : HK2 antibody detects endogenous levels of total HK2.
Immunogen : Purified recombinant fragment of human HK2 expressed in E. Coli.
Storage : Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human
Prediction :
Application : WB,IHC,ELISA,FCM
Dilution : ELISA 1 : 10000, WB 1 : 500-1 : 2000, IHC 1 : 200-1 : 1000, FCM 1 : 200-1 : 400
Conj µgate : Unconj µgated
RRID : AB_2833836
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide :
Des# Blocking Peptide :
Blocking Peptide Price : $549 CAD

Volume : 100 µL
Clone Number :
Aliases : HKI, HXK1 HK1-ta, HK1-tb, HK1-tc
Product Type : Monoclonal Antibody
Immunogen Species : Human
UniProt ID : P19367
Immunogen : Purified recombinant humanHK1 protein fragments expressed in E.coli.
Raised in : Mouse
Species Reactivity : Human,Dog,Pig,Mouse,Rat
Tested Applications : ELISA,WB,ICC;Recommended dilution : WB : 1 : 500-1 : 5000,ICC : 1 : 50-1 : 200
Background : Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria.
Clonality : Monoclonal
Isotype : IgG1
Purification Method : Affinity purified
Conj µgate : Non-conj µgated
Buffer : Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HK1
Research Areas : Cancer?Cardiovasc µLar;Metabolism;Signal transduction

Volume : 50 µg
Clone Number :
Aliases : BB404130 antibody; Brain form hexokinase antibody; dea antibody; DrHXK1 antibody; EC 2.7.1.1 antibody; Glycolytic enzyme antibody; HEXOKIN antibody; hexokinase I antibody; Hexokinase PI antibody; Hexokinase type I antibody; Hexokinase; tumor isozyme antibody; Hexokinase-1 antibody; Hexokinase-A antibody; HK I antibody; HK1 antibody; HK1 tb antibody; HK1 tc antibody; Hk1-s antibody; HK1-ta antibody; HK1-tb antibody; HK1-tc antibody; HKD antibody; HKI antibody; HMSNR antibody; HXK1 antibody; HXK1_HUMAN antibody; im : 7148527 antibody; mHk1-s antibody; wu : fc09d08 antibody; wu : fc16e02 antibody; wu : fc21e02 antibody; wu : fq14b11 antibody; zgc : 55790 antibody; zgc : 77618 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P19367
Immunogen : Recombinant Human Hexokinase-1 protein (13-475AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background :
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HK1
Research Areas : Cancer; Cardiovasc µLar; Metabolism; Signal transduction

Volume : 50 µg
Clone Number :
Aliases : BB404130 antibody; Brain form hexokinase antibody; dea antibody; DrHXK1 antibody; EC 2.7.1.1 antibody; Glycolytic enzyme antibody; HEXOKIN antibody; hexokinase I antibody; Hexokinase PI antibody; Hexokinase type I antibody; Hexokinase; tumor isozyme antibody; Hexokinase-1 antibody; Hexokinase-A antibody; HK I antibody; HK1 antibody; HK1 tb antibody; HK1 tc antibody; Hk1-s antibody; HK1-ta antibody; HK1-tb antibody; HK1-tc antibody; HKD antibody; HKI antibody; HMSNR antibody; HXK1 antibody; HXK1_HUMAN antibody; im : 7148527 antibody; mHk1-s antibody; wu : fc09d08 antibody; wu : fc16e02 antibody; wu : fc21e02 antibody; wu : fq14b11 antibody; zgc : 55790 antibody; zgc : 77618 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P19367
Immunogen : Recombinant Human Hexokinase-1 protein (13-475AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background :
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HK1
Research Areas : Cancer; Cardiovasc µLar; Metabolism; Signal transduction

Volume : 50 µg
Clone Number :
Aliases : BB404130 antibody; Brain form hexokinase antibody; dea antibody; DrHXK1 antibody; EC 2.7.1.1 antibody; Glycolytic enzyme antibody; HEXOKIN antibody; hexokinase I antibody; Hexokinase PI antibody; Hexokinase type I antibody; Hexokinase; tumor isozyme antibody; Hexokinase-1 antibody; Hexokinase-A antibody; HK I antibody; HK1 antibody; HK1 tb antibody; HK1 tc antibody; Hk1-s antibody; HK1-ta antibody; HK1-tb antibody; HK1-tc antibody; HKD antibody; HKI antibody; HMSNR antibody; HXK1 antibody; HXK1_HUMAN antibody; im : 7148527 antibody; mHk1-s antibody; wu : fc09d08 antibody; wu : fc16e02 antibody; wu : fc21e02 antibody; wu : fq14b11 antibody; zgc : 55790 antibody; zgc : 77618 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P19367
Immunogen : Recombinant Human Hexokinase-1 protein (13-475AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background :
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Biotin
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HK1
Research Areas : Cancer; Cardiovasc µLar; Metabolism; Signal transduction

Volume : 100 µL
Host : Mouse
Clonality : Monoclonal
Isotype : IgG1
GeneName : HK1
GeneID : 3098
Uniprot : P19367
Specificity : HK1 antibody detects endogenous levels of total HK1.
Immunogen : Purified recombinant fragment of human HK1 expressed in E. Coli.
Storage : Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse,Rat
Prediction :
Application : WB,IHC,IF/ICC,ELISA
Dilution : ELISA 1 : 10000, WB 1 : 500-1 : 2000, IHC 1 : 200-1 : 1000, IF/ICC 1 : 200-1 : 1000
Conj µgate : Unconj µgated
RRID : AB_2833832
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide :
Des# Blocking Peptide :
Blocking Peptide Price : $549 CAD

Volume : 50 µg
Clone Number :
Aliases : HJURP antibody; FAKTS antibody; FLEG1 antibody; URLC9 antibody; Holliday junction recognition protein antibody; 14-3-3-associated AKT substrate antibody; Fetal liver-expressing gene 1 protein antibody; Up-reg µLated in lung cancer 9 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q8NCD3
Immunogen : Recombinant Human Holliday junction recognition protein (1-748AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly syntheVolumed CENPA molec µLes into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HJURP
Research Areas : Others

Volume : 50 µg
Clone Number :
Aliases : HJURP antibody; FAKTS antibody; FLEG1 antibody; URLC9 antibody; Holliday junction recognition protein antibody; 14-3-3-associated AKT substrate antibody; Fetal liver-expressing gene 1 protein antibody; Up-reg µLated in lung cancer 9 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q8NCD3
Immunogen : Recombinant Human Holliday junction recognition protein (1-748AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background : Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly syntheVolumed CENPA molec µLes into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HJURP
Research Areas : Others

Volume : 50 µg
Clone Number :
Aliases : HJURP antibody; FAKTS antibody; FLEG1 antibody; URLC9 antibody; Holliday junction recognition protein antibody; 14-3-3-associated AKT substrate antibody; Fetal liver-expressing gene 1 protein antibody; Up-reg µLated in lung cancer 9 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q8NCD3
Immunogen : Recombinant Human Holliday junction recognition protein (1-748AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly syntheVolumed CENPA molec µLes into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Biotin
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HJURP
Research Areas : Others

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : HJURP
GeneID : 55355
Uniprot : Q8NCD3
Specificity : HJURP Antibody detects endogenous levels of total HJURP.
Immunogen : A syntheVolumed peptide derived from human HJURP, corresponding to a region within C-terminal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human
Prediction : Pig,Horse,Dog
Application : WB,ELISA(peptide)
Dilution : WB 1 : 1000-3000, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2842138
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : DF8942-BP
Des# Blocking Peptide : HJURP Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : HIVEP3
GeneID : 59269
Uniprot : Q5T1R4
Specificity : HIVEP3 Antibody detects endogenous levels of HIVEP3.
Immunogen : A syntheVolumed peptide derived from human HIVEP3.
Storage : Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Reactivity : Human,Mouse
Prediction :
Application : ELISA(peptide)
Dilution : ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID :
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : DF15088-BP
Des# Blocking Peptide : HIVEP3 Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : HIVEP1
GeneID : 3096
Uniprot : P15822
Specificity : HIVEP1 Antibody detects endogenous levels of HIVEP1.
Immunogen : A syntheVolumed peptide derived from human HIVEP1.
Storage : Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Reactivity : Human,Mouse
Prediction :
Application : ELISA(peptide)
Dilution : ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID :
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : DF15411-BP
Des# Blocking Peptide : HIVEP1 Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 50?l
Clone Number : 1G2E4
Aliases : "Histone H3.1 Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3C1 H3FA, HIST1H3A AND, H3C2, H3FL, HIST1H3B AND, H3C3, H3FC HIST1H3C AND, H3C4, H3FB, HIST1H3D AND, H3C6, H3FD, HIST1H3E AND, H3C7, H3FI, HIST1H3F AND, H3C8, H3FH, HIST1H3G AND, H3C10, H3FK, HIST1H3H AND, H3C11 H3FF, HIST1H3I AND, H3C12, H3FJ, HIST1H3J"
Product Type : Monoclonal Antibody
Immunogen Species :
UniProt ID :
Immunogen : KDIQLARRIRGERA synthetic peptide conj µgate to KLH
Raised in : Mouse
Species Reactivity : Human, Rat, Rabbit, Mouse
Tested Applications : ELISA, WB, IHC, IP;
Background : Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cell µLar machineries which require DNA as a template. Histones thereby play a central role in transcription reg µLation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is reg µLated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Clonality : Monoclonal
Isotype : IgG2b
Purification Method : Protein A purified
Conj µgate : Non-conj µgated
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HistoneH3
Research Areas : Others

Volume : 100 µL
Clone Number :
Aliases : H4; H4/n; H4F2; H4FN; FO108; HIST2H4
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic peptide corresponding to the amino terminus of histone H4 in which Arg3 is di-methylated.
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 50-1 : 200
Background :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted thro µgh the interaction of a linker histone, H1 with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : H3t; H3.4; H3/g; H3FT;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding Arg3 of Human histone H4
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IF, IP, ChIP; WB : 1 : 500-1 : 2000, IF : 1 : 50-1 : 200, IP : 1 : 50-1 : 200, ChIP : 1 : 50-1 : 200
Background :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted thro µgh the interaction of a linker histone, H1 with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : H4; H4/n; H4F2; H4FN; FO108; HIST2H4
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic peptide corresponding to the amino terminus of histone H4 in which Arg3 is mono-methylated.
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IF, IP; WB : 1 : 500-1 : 2000, IF : 1 : 50-1 : 200, IP : 1 : 50-1 : 200
Background :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted thro µgh the interaction of a linker histone, H1 with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : H4; H4/n; H4F2; H4FN; FO108; HIST2H4
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding K20 of Human histone H4
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IF, IP, ChIP; WB : 1 : 500-1 : 2000, IF : 1 : 50-1 : 200, IP : 1 : 50-1 : 200, ChIP : 1 : 50-1 : 200
Background :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted thro µgh the interaction of a linker histone, H1 with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : H4; H4/n; H4F2; H4FN; FO108; HIST2H4
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding K20 of Human histone H4
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IF, IP, ChIP; WB : 1 : 500-1 : 2000, IF : 1 : 50-1 : 200, IP : 1 : 50-1 : 200, ChIP : 1 : 50-1 : 200
Background :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted thro µgh the interaction of a linker histone, H1 with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : H4; H4/n; H4F2; H4FN; FO108; HIST2H4
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic peptide corresponding to the amino terminus of histone H4 in which Lys20 is mono-methylated.
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IF, IP, ChIP; WB : 1 : 500-1 : 2000, IF : 1 : 50-1 : 200, IP : 1 : 50-1 : 200, ChIP : 1 : 50-1 : 200
Background :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted thro µgh the interaction of a linker histone, H1 with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; HIST4H4
GeneID : 121504,554313,8294,8359,8360,83618362,8363,8364,8365,8366,8367,8368,8370
Uniprot : P62805
Specificity : Histone H4 Antibody detects endogenous levels of total Histone H4.
Immunogen : A syntheVolumed peptide derived from human Histone H4, corresponding to a region within N-terminal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse,Rat
Prediction : Pig,Bovine,Xenopus
Application : WB,IHC,IF/ICC,ELISA(peptide)
Dilution : WB 1 : 500-1 : 1000, IHC : 1 : 50-1 : 200, IF/ICC 1 : 100-1 : 500, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2837207
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : DF4843-BP
Des# Blocking Peptide : Histone H4 Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; 3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding R8of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IF, IP; WB : 1 : 500-1 : 2000, IF : 1 : 50-1 : 200, IP : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding R8 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding R8 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF, ChIP; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 50-1 : 200, ChIP : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : HIST3H3
GeneID : 8290
Uniprot : Q16695
Specificity : Histone H3R2me2s Antibody detects endogenous levels of total Histone H3R2me2s.
Immunogen : A syntheVolumed peptide derived from human Histone H3R2me2s
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse
Prediction :
Application : WB,IF/ICC,IP,ELISA(peptide)
Dilution : WB 1 : 500-1 : 2000, IF/ICC 1 : 50–1 : 200, IP 1 : 50–1 : 200, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID :
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : DF6951-BP
Des# Blocking Peptide : Histone H3R2me2s Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding R2 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j;H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding Arg26 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IF; WB : 1 : 500-1 : 2000, IF : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding Arg26 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IF, IP, ChIP; WB : 1 : 500-1 : 2000, IF : 1 : 50-1 : 200, IP : 1 : 50-1 : 200, ChIP : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding R26 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding Arg17 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding Arg17 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IF, IP, ChIP; WB : 1 : 500-1 : 2000, IF : 1 : 50-1 : 200, IP : 1 : 50-1 : 200, ChIP : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic mono-methylatedpeptide corresponding to residues surrounding Arg17 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding K9 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF, IP, ChIP; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 50-1 : 200, IP : 1 : 50-1 : 200, ChIP : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) ChemBiol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J BiolChem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding K9 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF, IP, ChIP; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 50-1 : 200, IP : 1 : 50-1 : 200, ChIP : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding K9 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF, IP, ChIP; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 50-1 : 200, IP : 1 : 50-1 : 200, ChIP : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding K79 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF, IP, ChIP; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 50-1 : 200, IP : 1 : 50-1 : 200, ChIP : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding K79 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF, IP, ChIP; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 50-1 : 200, IP : 1 : 50-1 : 200, ChIP : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding K4 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF, IP, ChIP; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 50-1 : 200, IP : 1 : 50-1 : 200, ChIP : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j;H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding K4 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF, IP, ChIP; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 50-1 : 200, IP : 1 : 50-1 : 200, ChIP : 1 : 50-1 : 200
Background : Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11). 1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.4. Cheung, P. et al. (2000) Cell 103, 263-71.5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding K4 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j;H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic tri-methylated peptide corresponding to residues surrounding K36of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IF, ChIP; WB : 1 : 500-1 : 2000, IF : 1 : 50-1 : 200, ChIP : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding K36 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding K36 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IF; WB : 1 : 500-1 : 2000, IF : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding K27 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF, IP, ChIP; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 50-1 : 200, IP : 1 : 50-1 : 200, ChIP : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
"
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding K27 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF, IP; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 50-1 : 200, IP : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic methylated peptide corresponding to residues surrounding K27 of Human histone H3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 50-1 : 200
Background :
Mod µLation of chromatin structure plays an important role in the reg µLation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stim µLi and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : H3t; H3.4; H3/g; H3FT;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic peptide of Human H3K14me3
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 20-1 : 50
Background :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted thro µgh the interaction of a linker histone, H1 with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : H3t; H3.4; H3/g; H3FT;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic peptide of Human H3K14me2
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 100, IF : 1 : 20-1 : 50
Background :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted thro µgh the interaction of a linker histone, H1 with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : H3t; H3.4; H3/g; H3FT;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : GeneID : 8290SwissProt : Q16695
Immunogen : A synthetic peptide of Human H3K14me1
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC, IF; WB : 1 : 500-1 : 2000, IHC : 1 : 50-1 : 200, IF : 1 : 20-1 : 50
Background :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted thro µgh the interaction of a linker histone, H1 with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST3H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µg
Purification : Immunogen affinity purified
Form : liquid
Purity : 95% as determined by SDS-PAGE
Host : Rabbit
Clonality : polyclonal Ab
Clone ID :
Isotype : IgG
Storage : PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.)
Background :
Immunogen : H3 histone, family 3A
Aliases : H3 histone, family 3A, H3.3A, H3.3B, H3F3, H3F3A, H3F3B, Histone H3.3
Observed MW : 15-17 kDa
Uniprot ID : P84243
Reactivity : Human, Mouse, Rat
Application : ELISA, WB, IHC
Recommended dilution : WB : 1 : 500-1 : 1000; IHC : 1 : 50-1 : 500
Gene ID :
Research Area : Epigenetics, Metabolism

Volume : 50 µL
Clone Number : 27F2
Aliases : Histone H3.1 Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, H3FA, AND, HIST1H3B, H3FL, AND, HIST1H3C, H3FC, AND, HIST1H3D, H3FB, AND, HIST1H3E, H3FD, AND, HIST1H3F, H3FI, AND, HIST1H3G, H3FH, AND, HIST1H3H, H3FK, AND, HIST1H3I, H3FF, AND, HIST1H3J, H3FJ
Product Type : Recombinant Antibody
Immunogen Species : Human
UniProt ID : P68431
Immunogen : A syntheVolumed peptide
Raised in :
Species Reactivity : Human
Tested Applications : ELISA, WB, IHC, IF, FC; Recommended dilution : WB : 1 : 500-1 : 5000, IHC : 1 : 50-1 : 500, IF : 1 : 30-1 : 200
Background : Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cell µLar machineries which require DNA as a template. Histones thereby play a central role in transcription reg µLation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is reg µLated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Clonality : Monoclonal
Isotype : Rabbit IgG
Purification Method : Affinity-chromatography
Conj µgate : Non-conj µgated
Buffer : Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST1H3A
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Clone Number :
Aliases : H3 histone family member E pseudogene antibody; H3 histone family; member A antibody; H3/A antibody; H31_HUMAN antibody; H3F3 antibody; H3FA antibody; Hist1h3a antibody; HIST1H3B antibody; HIST1H3C antibody; HIST1H3D antibody; HIST1H3E antibody; HIST1H3F antibody; HIST1H3G antibody; HIST1H3H antibody; HIST1H3I antibody; HIST1H3J antibody; HIST3H3 antibody; histone 1; H3a antibody; Histone cluster 1; H3a antibody; Histone H3 3 pseudogene antibody; Histone H3.1 antibody; Histone H3/a antibody; Histone H3/b antibody; Histone H3/c antibody; Histone H3/d antibody; Histone H3/f antibody; Histone H3/h antibody; Histone H3/i antibody; Histone H3/j antibody; Histone H3/k antibody; Histone H3/l antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P68431
Immunogen : Peptide sequence around aa.8~12 (R-K-S-T-G) derived from Human Histone H3.1.
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IF; WB : 1 : 500-1 : 1000, IF : 1 : 100-1 : 200
Background : Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cell µLar machineries which require DNA as a template. Histones thereby play a central role in transcription reg µLation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is reg µLated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
Cheung, P. et al. (2000) Cell 103, 263-71.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conj µgates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Conj µgate : Non-conj µgated
Buffer : Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST1H3A
Research Areas : Epigenetics and Nuclear Signaling

Volume : 50 µg
Clone Number :
Aliases : HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/
Product Type : Monoclonal Antibody
Immunogen Species : Human
UniProt ID : P68431/Q71DI3/P84243
Immunogen : Recombinant Protein
Raised in : Mouse
Species Reactivity : Human,Mouse,Rat
Tested Applications : ELISA,WB;Recommended dilution : WB : 1 : 500-1 : 5000
Background :
Clonality : Monoclonal
Isotype : IgG
Purification Method : The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Conj µgate : Non-conj µgated
Buffer : PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : Histone H3
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : HIST3H3
GeneID : 8290
Uniprot : Q16695
Specificity : Histone H3 Antibody detects endogenous levels of total Histone H3.
Immunogen : A syntheVolumed peptide derived from human Histone H3, corresponding to a region within N-terminal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse,Rat
Prediction : Horse,Sheep,Rabbit
Application : WB,IHC,IF/ICC,IP,ChIP,ELISA(peptide)
Dilution : WB 1 : 500-1 : 2000, IHC 1 : 50-1 : 200, IF/ICC 1 : 50-1 : 200, IP 1 : 50-1 : 200, CHIP 1 : 50-1 : 200, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2838891
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : DF6932-BP
Des# Blocking Peptide : Histone H3 Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 100 µL
Clone Number :
Aliases : H3 histone family member E pseudogene antibody; H3 histone family; member A antibody; H3/A antibody; H31_HUMAN antibody; H3F3 antibody; H3FA antibody; Hist1h3a antibody; HIST1H3B antibody; HIST1H3C antibody; HIST1H3D antibody; HIST1H3E antibody; HIST1H3F antibody; HIST1H3G antibody; HIST1H3H antibody; HIST1H3I antibody; HIST1H3J antibody; HIST3H3 antibody; histone 1; H3a antibody; Histone cluster 1; H3a antibody; Histone H3 3 pseudogene antibody; Histone H3.1 antibody; Histone H3/a antibody; Histone H3/b antibody; Histone H3/c antibody; Histone H3/d antibody; Histone H3/f antibody; Histone H3/h antibody; Histone H3/i antibody; Histone H3/j antibody; Histone H3/k antibody; Histone H3/l antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P68431
Immunogen : Peptide sequence around aa.26-30(A-R-K-S-A) derived from Human Histone H3.
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB; WB : 1 : 500-1 : 1000
Background :
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cell µLar machineries which require DNA as a template. Histones thereby play a central role in transcription reg µLation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is reg µLated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Choi H.S., Choi B.Y., Cho Y.-Y., Zhu F., Bode A.M., Dong Z.J. Biol. Chem. 280 : 13545-13553(2005)
Tan M., Luo H., Lee S., Jin F., Yang J.S., Montellier E., Buchou T., Cheng Z., Rousseaux S., Rajagopal N., Lu Z., Ye Z., Zhu Q., Wysocka J., Ye Y., Khochbin S., Ren B., Zhao Y.
Cell 146 : 1016-1028(2011)
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conj µgates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Conj µgate : Non-conj µgated
Buffer : Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST1H3A
Research Areas : Epigenetics and Nuclear Signaling

Volume : 50 µL
Clone Number : 4G10
Aliases : Histone H2B type 1-K, H2B K, HIRA-interacting protein 1 HIST1H2BK, H2BFT, HIRIP1
Product Type : Recombinant Antibody
Immunogen Species : Human
UniProt ID : O60814
Immunogen : A syntheVolumed peptide
Raised in :
Species Reactivity : Human
Tested Applications : ELISA, WB, IHC; Recommended dilution : WB : 1 : 5000-1 : 10000, IHC : 1 : 50-1 : 500
Background : Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cell µLar machineries which require DNA as a template. Histones thereby play a central role in transcription reg µLation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is reg µLated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Clonality : Monoclonal
Isotype : Rabbit IgG
Purification Method : Affinity-chromatography
Conj µgate : Non-conj µgated
Buffer : Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST1H2BK
Research Areas : Epigenetics and Nuclear Signaling

Volume : 50 µg
Clone Number :
Aliases : HIST1H2BB; H2BFF; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B/f
Product Type : Monoclonal Antibody
Immunogen Species : Human
UniProt ID : P33778
Immunogen : Synthetic Peptide
Raised in : Mouse
Species Reactivity : Human,Mouse,Rat
Tested Applications : ELISA,WB;Recommended dilution : WB : 1 : 500-1 : 5000
Background :
Clonality : Monoclonal
Isotype : IgG
Purification Method : The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Conj µgate : Non-conj µgated
Buffer : PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : Histone H2B
Research Areas : Epigenetics and Nuclear Signaling

Volume : 50 µg
Clone Number :
Aliases : Histone H2B antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Cairina moschata (Muscovy duck)
UniProt ID : P14001
Immunogen : Recombinant Crocodylus niloticus Histone H2B protein (2-93AA)
Raised in : Rabbit
Species Reactivity : Cairina moschata
Tested Applications : ELISA
Background : Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cell µLar machineries which require DNA as a template. Histones thereby play a central role in transcription reg µLation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is reg µLated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names :
Research Areas : Others

Volume : 50 µg
Clone Number :
Aliases : Histone H2B antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Cairina moschata (Muscovy duck)
UniProt ID : P14001
Immunogen : Recombinant Crocodylus niloticus Histone H2B protein (2-93AA)
Raised in : Rabbit
Species Reactivity : Cairina moschata
Tested Applications :
Background : Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cell µLar machineries which require DNA as a template. Histones thereby play a central role in transcription reg µLation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is reg µLated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names :
Research Areas : Others

Volume : 50 µg
Clone Number :
Aliases : Histone H2B antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Cairina moschata (Muscovy duck)
UniProt ID : P14001
Immunogen : Recombinant Crocodylus niloticus Histone H2B protein (2-93AA)
Raised in : Rabbit
Species Reactivity : Cairina moschata
Tested Applications : ELISA
Background : Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cell µLar machineries which require DNA as a template. Histones thereby play a central role in transcription reg µLation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is reg µLated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Biotin
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names :
Research Areas : Others

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : HIST1H2BC; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BI,HIST1H2BJ,HIST1H2BB
GeneID : 3017,8339,8343,8344,8346,8347,8970,3018
Uniprot : P33778/P62807/P58876/P06899/O60814
Specificity : Histone H2B Antibody detects endogenous levels of total Histone H2B.
Immunogen : A syntheVolumed peptide derived from human Histone H2B, corresponding to a region within N-terminal amino acids.
Storage : Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Reactivity : Human,Mouse,Rat
Prediction : Pig,Bovine,Horse,Sheep,Rabbit,Dog
Application : WB,ELISA(peptide)
Dilution : WB 1 : 500-1 : 2000, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2839899
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : DF2693-BP
Des# Blocking Peptide : Histone H2B Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : H2AFZ
GeneID : 3015
Uniprot : P0C0S5
Specificity : Histone H2AZ Antibody detects endogenous levels of total Histone H2AZ.
Immunogen : A syntheVolumed peptide derived from human Histone H2AZ, corresponding to a region within N-terminal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse,Rat
Prediction : Bovine,Sheep,Dog,Chicken,Xenopus
Application : WB,ELISA(peptide)
Dilution : WB 1 : 500-1 : 2000, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2844675
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : AF4666-BP
Des# Blocking Peptide : Histone H2AZ Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 100 µL
Clone Number :
Aliases : H2A histone family member X antibody; H2A histone family member X antibody; H2A.FX antibody; H2A.X antibody; H2a/x antibody; H2AFX antibody; H2AX antibody; H2AX_HUMAN antibody; Histone H2A.X antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P16104
Immunogen : SyntheVolumed peptide derived from internal of Human Histone H2AX.
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA, WB, IF; WB : 1 : 500-1 : 3000, IF : 1 : 100-1 : 500
Background : Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cell µLar machineries which require DNA as a template. Histones thereby play a central role in transcription reg µLation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is reg µLated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

The MGC Project Team; Genome Res. 14 : 2121-2127(2004).
Rogakou E.P., J. Biol. Chem. 273 : 5858-5868(1998).
Rogakou E.P., J. Biol. Chem. 275 : 9390-9395(2000).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conj µgate : Non-conj µgated
Buffer : Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : H2AFX
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : HIST1H2AB; HIST1H2AE
GeneID : 3012,8335
Uniprot : P04908
Specificity : Histone H2A.Z Antibody detects endogenous levels of total Histone H2A.Z.
Immunogen : A syntheVolumed peptide derived from human Histone H2A.Z, corresponding to a region within C-terminal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse,Rat
Prediction :
Application : WB,IHC,IF/ICC,ELISA(peptide)
Dilution : WB 1 : 1000-3000, IHC 1 : 200, IF/ICC, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2840916
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : DF2930-BP
Des# Blocking Peptide : Histone H2A.Z Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 100 µg
Purification : Immunogen affinity purified
Form : liquid
Purity : 95% as determined by SDS-PAGE
Host : Rabbit
Clonality : polyclonal Ab
Clone ID :
Isotype : IgG
Storage : PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20? for 12 months (Avoid repeated freeze / thaw cycles.)
Background : Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molec µLes of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1 interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts thro µgh the use of the conserved stem-loop termination motif, and the polyA addition motif.
Immunogen : H2A histone family, member X
Aliases : H2A histone family, member X, H2A.X, H2A/X, H2AFX, H2AX, Histone H2A.x
Observed MW : 16 kDa
Uniprot ID : P16104
Reactivity : Human, Mouse, Rat
Application : ELISA, WB, IHC, IF
Recommended dilution : WB : 1 : 500 - 1 : 2000; IHC : 1 : 100 - 1 : 200; IF : 1 : 50 - 1 : 200
Gene ID : 3014
Research Area : Epigenetics, Metabolism, Developmental biology

Volume : 100 µL
Clone Number :
Aliases : H2A histone family member X antibody; H2A histone family member X antibody; H2A.FX antibody; H2A.X antibody; H2a/x antibody; H2AFX antibody; H2AX antibody; H2AX_HUMAN antibody; Histone H2A.X antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P16104
Immunogen : Peptide sequence around aa.137~141 (Q-A-S-Q-E) derived from Human Histone H2A.X.
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA, WB, IHC, IF; WB : 1 : 500-1 : 1000, IHC : 1 : 50-1 : 200, IF : 1 : 100-1 : 200
Background : Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cell µLar machineries which require DNA as a template. Histones thereby play a central role in transcription reg µLation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is reg µLated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation

Yaneva M, et al. (2005) Nucleic Acids Res. 33(16) : 5320-5330.
Tsukuda T, et al.(2006) Nature. Author manuscript; available in PMC 2006 March 6
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conj µgates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Conj µgate : Non-conj µgated
Buffer : Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : H2AFX
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : H2AFB1
GeneID : 474382
Uniprot : P0C5Y9
Specificity : Histone H2A-Bbd Antibody detects endogenous levels of total Histone H2A-Bbd.
Immunogen : A syntheVolumed peptide derived from human Histone H2A-Bbd, corresponding to a region within the internal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human
Prediction :
Application : WB,ELISA(peptide)
Dilution : WB 1 : 500-1 : 2000, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2846485
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : DF13466-BP
Des# Blocking Peptide : Histone H2A-Bbd Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 50 µL
Clone Number : 1H12
Aliases : Histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a, Histone H2A/m, HIST1H2AB, H2AFM, AND, HIST1H2AE, H2AFA
Product Type : Recombinant Antibody
Immunogen Species : Human
UniProt ID : P04908
Immunogen : A syntheVolumed peptide
Raised in :
Species Reactivity : Human
Tested Applications : ELISA, WB, IF; Recommended dilution : WB : 1 : 500-1 : 5000, IF : 1 : 30-1 : 200
Background : Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cell µLar machineries which require DNA as a template. Histones thereby play a central role in transcription reg µLation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is reg µLated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Clonality : Monoclonal
Isotype : Rabbit IgG
Purification Method : Affinity-chromatography
Conj µgate : Non-conj µgated
Buffer : Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : HIST1H2AB
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : HIST1H2AA,HIST1H2AB; HIST1H2AE,HIST1H2AC,HIST2H2AB,HIST1H2AD,HIST1H2AG; HIST1H2AI; HIST1H2AK; HIST1H2AL; HIST1H2AM,HIST2H2AC,HIST2H2AA3; HIST2H2AA4,HIST1H2AJ,HIST3H2A
GeneID : 221613,3012,8335,8334,317772,3013,8329,8330,8332,8336,8969,8338,723790,8337,833192815
Uniprot : P04908/P0C0S8/P20671/Q16777/Q6FI13/Q7L7L0/Q8IUE6/Q93077/Q96QV6/Q99878
Specificity : Histone H2A Antibody detects endogenous levels of total Histone H2A.
Immunogen : A syntheVolumed peptide derived from human Histone H2A, corresponding to a region within C-terminal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse,Rat
Prediction :
Application : WB,IHC,ELISA(peptide)
Dilution : IHC 1 : 50-1 : 200, WB 1 : 500-1 : 2000, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2834406
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : AF0875-BP
Des# Blocking Peptide : Histone H2A Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : H1FX
GeneID : 8971
Uniprot : Q92522
Specificity : Histone H1X Antibody detects endogenous levels of total Histone H1X.
Immunogen : A syntheVolumed peptide derived from human Histone H1X, corresponding to a region within N-terminal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human
Prediction : Pig,Bovine,Sheep,Xenopus
Application : WB,ELISA(peptide)
Dilution : WB 1 : 1000-3000, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2842052
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : DF8855-BP
Des# Blocking Peptide : Histone H1X Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : HIST1H1B
GeneID : 3009
Uniprot : P16401
Specificity : Histone H1.5 Antibody detects endogenous levels of total Histone H1.5.
Immunogen : A syntheVolumed peptide derived from human Histone H1.5, corresponding to a region within N-terminal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse,Rat
Prediction :
Application : WB,IHC,IF/ICC,ELISA(peptide)
Dilution : WB 1 : 500-1 : 2000, IF/ICC 1 : 100-1 : 500, IHC 1 : 50-1 : 200, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2846558
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : DF13539-BP
Des# Blocking Peptide : Histone H1.5 Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName :
GeneID :
Uniprot : P10412/P16402
Specificity : Histone H1.3 Antibody detects endogenous levels of total Histone H1.3.
Immunogen : A syntheVolumed peptide derived from human Histone H1.3.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse
Prediction :
Application : WB,ELISA(peptide)
Dilution : WB 1 : 500-1 : 2000, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID :
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : AF0082-BP
Des# Blocking Peptide : Histone H1.3 Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : HIST1H1C
GeneID : 3006
Uniprot : P16403
Specificity : Histone H1.2 Antibody detects endogenous levels of total Histone H1.2.
Immunogen : A syntheVolumed peptide derived from human Histone H1.2, corresponding to a region within N-terminal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse
Prediction : Bovine,Sheep,Dog
Application : WB,IHC
Dilution : WB 1 : 500-1 : 2000, IHC 1 : 50-1 : 200
Conj µgate : Unconj µgated
RRID : AB_2845084
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : DF12279-BP
Des# Blocking Peptide : Histone H1.2 Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : H1F0
GeneID : 3005
Uniprot : P07305
Specificity : Histone H1.0 Antibody detects endogenous levels of total Histone H1.0.
Immunogen : A syntheVolumed peptide derived from human Histone H1.0, corresponding to a region within the internal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse,Rat
Prediction : Pig,Bovine,Sheep
Application : WB,IF/ICC,ELISA(peptide)
Dilution : WB 1 : 500-1 : 1000, IF/ICC 1 : 100-1 : 500, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2835456
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : DF3073-BP
Des# Blocking Peptide : Histone H1.0 Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 100 µg
Purification : Immunogen affinity purified
Form : liquid
Purity : 95% as determined by SDS-PAGE
Host : Rabbit
Clonality : polyclonal Ab
Clone ID :
Isotype : IgG
Storage : PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.)
Background :
Immunogen : histone cluster 1 H1a
Aliases :
Observed MW : 28kd,30kd
Uniprot ID : Q02539
Reactivity : Human, Mouse, Rat
Application : ELISA, WB, IP
Recommended dilution : WB : 1 : 500-1 : 2000; IP : 1 : 200-1 : 2000
Gene ID :
Research Area : Epigenetics, Metabolism, Developmental biology