Volume : 50 µg
Clone Number :
Aliases : GBA2 antibody; KIAA1605 antibody; SPG46 antibody; AD035Non-lysosomal glucosylceramidase antibody; NLGase antibody; EC 3.2.1.45 antibody; Beta-glucocerebrosidase 2 antibody; Beta-glucosidase 2 antibody; Bile acid beta-glucosidase GBA2 antibody; Bile acid glucosyl transferase GBA2 antibody; Cholesterol glucosyltransferase GBA2 antibody; EC 2.4.1.- antibody; Cholesteryl-beta-glucosidase GBA2 antibody; EC 3.2.1.104 antibody; Glucosylceramidase 2 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q9HCG7
Immunogen : Recombinant Human Non-lysosomal glucosylceramidase protein (656-867AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : Non-lysosomal glucosylceramidase that catalyzes the conversion of glucosylceramide (GlcCer) to free glucose and ceramide. Involved in sphingomyelin generation and prevention of glycolipid accum µLation. May also catalyze the hydrolysis of bile acid 3-O-glucosides, however, the relevance of such activity is unclear in vivo. Plays a role in central nevous system development. Required for proper formation of motor neuron axons.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Biotin
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GBA2
Research Areas : Metabolism; Signal transduction

Volume : 100 µg
Purification : Immunogen affinity purified
Form : liquid
Purity : 95% as determined by SDS-PAGE
Host : Rabbit
Clonality : polyclonal Ab
Clone ID :
Isotype : IgG
Storage : PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.)
Background : Non-lysosomal glucosylceramidase that catalyzes the conversion of glucosylceramide(GlcCer) to free glucose and ceramide. Involved in sphingomyelin generation and prevention of glycolipid accum µLation. May also catalyze the hydrolysis of bile acid 3-O-glucosides, however, the relevance of such activity is unclear in vivo. Plays a role in central nevous system development. Required for proper formation of motor neuron axons.
Immunogen : glucosidase, beta(bile acid) 2
Aliases : KIAA1605
Observed MW :
Uniprot ID : Q9HCG7
Reactivity : Human, Mouse, Rat
Application : ELISA
Recommended dilution :
Gene ID : 57704
Research Area : Metabolism

Volume : 50 µg
Clone Number :
Aliases : GbaLysosomal acid glucosylceramidase antibody; Lysosomal acid GCase antibody; EC 3.2.1.45 antibody; Acid beta-glucosidase antibody; Beta-glucocerebrosidase antibody; Cholesterol glucosyltransferase antibody; SGTase antibody; EC 2.4.1.- antibody; Cholesteryl-beta-glucosidase antibody; EC 3.2.1.104 antibody; D-glucosyl-N-acylsphingosine glucohydrolase antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Mus musc µLus (Mouse)
UniProt ID : P17439
Immunogen : Recombinant Mouse Glucosylceramidase protein (20-515AA)
Raised in : Rabbit
Species Reactivity : Mouse
Tested Applications : ELISA
Background : extracell µLar exosome, extracell µLar space, lysosomal lumen, lysosomal membrane, glucosylceramidase activity, hydrolase activity, receptor binding, cell µLar response to starvation, cell µLar response to tumor necrosis factor, ceramide biosynthetic process
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : Gba
Research Areas : Others

Volume : 50 µg
Clone Number :
Aliases : GbaLysosomal acid glucosylceramidase antibody; Lysosomal acid GCase antibody; EC 3.2.1.45 antibody; Acid beta-glucosidase antibody; Beta-glucocerebrosidase antibody; Cholesterol glucosyltransferase antibody; SGTase antibody; EC 2.4.1.- antibody; Cholesteryl-beta-glucosidase antibody; EC 3.2.1.104 antibody; D-glucosyl-N-acylsphingosine glucohydrolase antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Mus musc µLus (Mouse)
UniProt ID : P17439
Immunogen : Recombinant Mouse Glucosylceramidase protein (20-515AA)
Raised in : Rabbit
Species Reactivity : Mouse
Tested Applications :
Background : extracell µLar exosome, extracell µLar space, lysosomal lumen, lysosomal membrane, glucosylceramidase activity, hydrolase activity, receptor binding, cell µLar response to starvation, cell µLar response to tumor necrosis factor, ceramide biosynthetic process
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : Gba
Research Areas : Others

Volume : 50 µg
Clone Number :
Aliases : GbaLysosomal acid glucosylceramidase antibody; Lysosomal acid GCase antibody; EC 3.2.1.45 antibody; Acid beta-glucosidase antibody; Beta-glucocerebrosidase antibody; Cholesterol glucosyltransferase antibody; SGTase antibody; EC 2.4.1.- antibody; Cholesteryl-beta-glucosidase antibody; EC 3.2.1.104 antibody; D-glucosyl-N-acylsphingosine glucohydrolase antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Mus musc µLus (Mouse)
UniProt ID : P17439
Immunogen : Recombinant Mouse Glucosylceramidase protein (20-515AA)
Raised in : Rabbit
Species Reactivity : Mouse
Tested Applications : ELISA
Background : extracell µLar exosome, extracell µLar space, lysosomal lumen, lysosomal membrane, glucosylceramidase activity, hydrolase activity, receptor binding, cell µLar response to starvation, cell µLar response to tumor necrosis factor, ceramide biosynthetic process
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Biotin
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : Gba
Research Areas : Others

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : GBA
GeneID : 2629
Uniprot : P04062
Specificity : GBA Antibody detects endogenous levels of total GBA.
Immunogen : A syntheVolumed peptide derived from human GBA, corresponding to a region within the internal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse
Prediction : Horse,Rabbit,Dog
Application : WB,IHC,ELISA(peptide)
Dilution : WB 1 : 1000-3000, IHC 1 : 50-1 : 200, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2841533
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : DF8237-BP
Des# Blocking Peptide : GBA Blocking peptide
Blocking Peptide Price : $549 CAD

GB-Workhorse™ Horizontal Electrophoresis Apparatus System is for running nucleic acid samples, such as DNA samples by electrophoresis. This system offers gel trays, comb and gel cell/tank for running buffers/gel tray, with attached electrical leads.

Technical specifications

Gel Volume(W×L) : 78×100mm
Sample volume : 10, 15.
No. of Samples : 10~30
Buffer Volume : ~400ml.
Dimension(L×W×H) : 365×105×55(mm)
Net weight : 0.6(kg)g)

Features :
• Abundant buffer not only guarantees cooling effects, but also keeps the pH Value stable during the whole experiment process.
• Unfolded electrodes make repair and parts replacement easy and quick.
• With the sample background color design on the tray, convenient to adding the sample.
• The transparent top lid effectively prevents liquid in the cell from volatilizing and avoids electricity leakage.
• Auto-switch-off when the lid is removed.

The product includes :
Body tank with electrode / 1
Gel tray (W×L) : 78×100mm 1
Gel box (W×L) : 78×100(mm) 1
Combs :
1.0mm thickness,10-wells 2
2.0mm thickness,10-wells 2
1.0mm thickness,15-wells 2
2.0mm thickness,15-wells 2
Electrical Cables / 1

10-30% OFF list price for the 50ml variant as part of our FALL Sale! On now until October 18th, 2024!
Unique genomic DNA remover is combined with TransScript® First-Strand cDNA Synthesis kit (AT311) to enable sim µLtaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds.
The users can optimize the reverse transcription primer, choosing between polydT and random 9-mers, as well as the primer concentration. In addition, the concentration of the transcriptase can also be optimized.
• Sim µLtaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• cDNA up to 12 kb.

Applications
M µLtiple copy and low copy gene detection

Storage
at -20 ℃ for two years

Product Contents

Component AT311-02 (50 rxns) AT311-03 (100 rxns) AT311-04 (500 rxns)
TransScript® RT/RI Enzyme Mix 50 μl 100 μl 5×100 μl
gDNA Remover 50 μl 100 μl 5×100 μl
2×TS Reaction Mix 500 μl 1 ml 5×1 ml
Random Primer(N9) (0.1 μg/μl) 50 μl 100 μl 5×100 μl
Anchored Oligo(dT)18 Primer (0.5 μg/μl) 50 μl 100 μl 5×100 μl
RNase-free Water 500 μl 1 ml 5 ml


Citations
Chen J , Ou Y , Yang Y , et al. KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing[J]. Nature, 2018,557(7706) : 585-589.
Yang Y , Liu B , Xu J , et al. Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency[J]. Cell, 2017, 169(2) : 243-257.e25.
Wu D , Qi T , Li W X , et al. Viral effector protein manip µLates host hormone signaling to attract insect vectors[J]. Cell Research, 2017, 27(3) : 402-415.
Shao X , Liu Y , Yu Q , et al. Numb reg µLates vesic µLar docking for homotypic fusion of early endosomes via membrane recruitment of Mon1b[J]. Cell Research, 2016,26(5) : 593-612.
Ma D , Hu Y , Yang C , et al. Genetic basis for gland µLar trichome formation in cotton[J]. Nature Communications, 2016, 7 : 10456.

Components R302-01 10,000 U
5x GB-Script™ IIIBuffer 500 μl
GB-Script™ III Reverse Transcriptase (200 U/ μl) 50 μl

This product delivers high efficiency of first strand cDNA synthesis. The 5× gDNA wiper Mix in the kit can quickly remove genomic DNA contamination at 42℃ for 2 min, which makes the res µLts more reliable and simplifies qPCR primer design process without the need to design primers across introns. The kit contains two separated primers : the reverse transcription primers Oligo (dT) 20 VN and Random hexamers, allowing users to choose primers flexibly for subsequent experiments as needed. The kit can syntheVolume f µLl-length cDNA (up to 20 kb) for downstream experiments such as cloning, and can also syntheVolume a highly uniform cDNA for qPCR quantification.

Components
R312-01 50rxn (20μl/rxn)
R312-02 100rxn (20μl/rxn)
RNasefreeddH2O
1 ml
1 ml
5× gDNAwiperMix
100 μl
200 μl
10x RTMix
100 μl
200 μl
GB-Script™ III EnzymeMix
100 μl
200 μl
Oligo (dT)20VN
50 μl
100 μl
RandomHexamers
50 μl
100 μl

This GB-R µLer™ Unstained Protein Ladder is a mixture of 12 unstained recombinant proteins covering a wide range of molec µLar weights from 10 to 200 kDa. The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25kDa and 85kDa protein bands serving as the two reference bands. The This GB-R µLer™ Unstained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocell µLose) and for approximating the Volume of proteins. The ladder is supplied in the gel loading buffer and is ready to use.
Figure Caption-3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Storage Buffer :
20 mM Tris-phosphate(pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol.
Quality Control :
Under s µggested conditions, Unstained Protein Ladder resolves 12 major bands in 4-12%Bis- Tris Gel(MES buffer) and after Western blotting to the nitrocell µLose membrane.
Storage :
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.
Note. The apparent molec µLar weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data sho µLd be considered for more accurate adjustment in different electrophoresis conditions.
All products are for research use only.
Caution :
Not intended for human or animal diagnostic or therapeutic uses.

The GB-R µLer™ 50bp ladder plus is ideal for determining the Volume of double-stranded DNA from 50 to 1000 base pairs. The ladder consists of 13 linear double-stranded fragments .The 250bp and 500bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 250bp and 500bp are 40ng. The 250bp and 500bp fragment are 100ng. This ladder is pre-mixed with loading dye and is ready to use.

Recommended Loading
2-5 µl/Lane

Concentration
Typical Bands 100 ng/5µl
Other Bands 40 ng/5µl

Advised Electrophoresis Condition
8 cm, 3% Agarose Gel, 0.5×TBE, 5 V/cm, 1 h

Contents（bp）
50、100、150、200、250、300、400、500、600、700、800、900、1000

Mix Concentration
128 ng/µl

Storage
Stable for 3 months at RT, for long-time storage, please store at -20℃.

The expected quantity of each DNA fragment in this marker solution is provided (assuming a 0.5 μg/ 5 μl load), so that you can use this information to estimate the concentration of a DNA fragment in a sample of similar Volume.
Features and Specifications

Volume Range (bp) : 100 to 1500 (1.5 kb)

# of bands : 11

Concentration (μg/ml) : 100

Recommended volume to load : 5 μl per well

Loading buffer Matrix : 10 mM Tris-HCl (pH 8), 10 mM EDTA, orange G, xylene cyanol FF and glycerol.

Storage : 25°C (for 6 months) and at -20°C (for 24 months).

GB-R µLer™ 100 bp Plus DNA Ladder is ideal for determining the Volume of double-stranded DNA from 100 to 3,000 base pairs. The ladder consists of 14 linear double-stranded fragments. The 500bp and 1200bp fragments are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µL loading, all fragments except 500bp and 1200bp are 40ng. The 500bp and 1200bp fragments are 100ng. This ladder is pre-mixed with loading dye and is ready to use.
Recommended Loading
2-5 µl/lane
Concentration
Typical Bands 100ng/5µl
Other Bands 40ng/5µl
Recommended Electrophoresis Condition
5 µl/lane, 8cm 1.7% Agarose Gel, 0.5×TBE, 5V/cm, 1h.
Contents(bp)
100,200,300,400,500,600,700,800,900,1000,1200,1500,2,000,3,000.
Storage
Stable for 3 months at RT, for long-time storage, please store at -20 ̊C.

The GB-R µLer™ 1kb Ladder Plus is ideal for determining the Volume of double-stranded DNA from 100 to 10,000 base pairs. The ladder consists of 15 linear double-stranded fragments. The 500bp and 3,000bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 500bp and 3,000bp are 40ng. The 500bp and 3,000bp fragment are 100ng.
This ladder is pre-mixed with loading dye and is ready to use. Ready to load to the gel. Gel loading dye already added.

Recommended Loading
2-5 µl/Lane

Concentration
Typical Bands 100 ng/5µl
Other Bands 40 ng/5µl

Advised Electrophoresis Condition
8 cm, 1% Agarose Gel, 1×TAE, 7V/cm, 45min

Contents（bp）
100、200 、300、400、500、700、1000、1500、2,000、3,000、4,000、5,000、6,000、8,000、10,000

Mix Concentration
144 ng/µl

Storage
Stable for 3 months at RT, for long-time storage, please store at -20℃.

The quantity of DNA fragment or band is provided for you to estimate the concentration of your bands from the sample.
Features and Specifications

Volume Range : 250 bp to 10 kb

Number of bands : 13 bands

Total Concentration : 100 μg/ml

Recommended Volume : 5 μl per well

Loading buffer : 10 mM Tris-HCl (pH 8), 10 mM EDTA, bromophenol blue and glycerol.

Storage : 25°C for 6 months and -20°C for 24 months.

20% OFF list price as part of our FALL Sale! On now until October 18th, 2024!
GB-Productivity™ Horizontal Electrophoresis Apparatus System is for running nucleic acid samples, such as DNA samples by electrophoresis. This system offers gel trays, comb and gel cell/tank for running buffers/gel tray, with attached electrical leads.

Technical specifications

Gel Volume(W×L) : 130×130mm; 78×100mm; 48×75mm.
Sample volume : 3, 5, 10, 15, 13, 26.
No. of Samples : 3~104
Buffer Volume : ~600ml.
Dimension(L×W×H) : 305×152×80(mm)
Net weight : 1.7(kg)

Features :
• Abundant buffer not only guarantees cooling effects, but also keeps the pH Value stable during the whole experiment process.
• Unfolded electrodes make repair and parts replacement easy and quick.
• With the fluorescent r µLer on the tray, can be observed directly into the µLtraviolet.
• With a special gel casting, preventing the gel leakage, operation is simple and convenient.
• The transparent top lid effectively prevents liquid in the cell from volatilizing and avoids electricity leakage.
• Auto-switch-off when the lid is removed.

The product includes :
Body tank with electrode / 1
Gel trays :
(W×L) : 130×130(mm) 1
(W×L) : 78×100(mm) 1
(W×L) : 48×75(mm) 2
Gel Casting / 1
Combs :
1.5mm thickness,3-wells 2
1.0mm thickness,5-wells 2
1.0mm thickness,10-wells 2
1.0mm thickness,13-wells 2
1.5mm thickness,13-wells 2
1.0mm thickness,15-wells 2
1.0mm thickness,26-wells 4
1.5mm thickness,26-wells 2
Ievel adjustment of leg button / 4
Electrical Cables / 1

20% OFF list price as part of our FALL Sale! On now until October 18th, 2024!
GB-Mini™ Horizontal Electrophoresis Apparatus System is for running nucleic acid samples, such as DNA samples by electrophoresis. This system offers gel trays, comb and gel cell/tank for running buffers/gel tray, with attached electrical leads.

Technical specifications

Gel Volume(W×L) : 48×75mm
Sample volume : 3, 5, 9.
No. of Samples : 3~18
Buffer Volume : ~150ml.
Dimension(L×W×H) : 250×90×50(mm)
Net weight : 0.4(kg)

Features :
• Abundant buffer not only guarantees cooling effects, but also keeps the pH Value stable during the whole experiment process.
• Unfolded electrodes make repair and parts replacement easy and quick.
• With the sample background color design on the tray, convenient to adding the sample.
• The transparent top lid effectively prevents liquid in the cell from volatilizing and avoids electricity leakage.
• Auto-switch-off when the lid is removed.

The product includes :
Body tank with electrode / 1
Gel tray (W×L)：48×75(mm) 1
Gel box (W×L)：48×75(mm) 1
Combs
1.0mm thickness,3-wells 2
1.5mm thickness,3-wells 2
1.0mm thickness,5-wells 2
1.5mm thickness,5-wells 2
1.0mm thickness,9-wells 2
1.5mm thickness,9-wells 2
Electrical Cables / 1

Buy 3 of ANY magnetic beads (smallest Volume) get 1 FREE (Add 4 to cart, get 1 of 4 FREE) as part of our FALL Sale! On now until October 18th, 2024!
The RNA Beads can be used to substitute the Agencourt® RNAClean® XP Beads (Beckman Co µLter, Cat.No. #A63987) in your current protocols.

Buy 3 of ANY magnetic beads (smallest Volume) get 1 FREE (Add 4 to cart, get 1 of 4 FREE) as part of our FALL Sale! On now until October 18th, 2024!
GB-Magic(TM) Protein A / G Matrix beads for immunoprecipitation are magnetic beads coupled with Protein A / G in high density by using proprietary nano- technology. Validated for Immunoprecipitation.

Buy 3 of ANY magnetic beads (smallest Volume) get 1 FREE (Add 4 to cart, get 1 of 4 FREE) as part of our FALL Sale! On now until October 18th, 2024!
GB-Magic(TM) Protein A Matrix beads for immunoprecipitation are magnetic beads coupled with Protein A in high density by using proprietary nano- technology. Validated for Immunoprecipitation.
Note, we also offer Protein A/G Beads. Please ask us or search for Protein A/G Immunoprecipitation Magnetic Beads Cat. #22202B-1 or 22202B-5.

Buy 3 of ANY magnetic beads (smallest Volume) get 1 FREE (Add 4 to cart, get 1 of 4 FREE) as part of our FALL Sale! On now until October 18th, 2024!
The usage of GB-Magic DNA Beads is the same as the AMPure® XP Beads (Beckman, Cat.No. #A63881), which is widely used in NGS library preparation. The yield and Volume distribution of the libraries prepared with GB-Magic DNA Beads are equivalent with those with AMPure® XP Beads. The cost-effective GB-Magic DNA Beads can replace the AMPure® XP Beads in your current protocols.
In addition to workflows in NGS for Volume selection and clean up, GB-Magic DNA Beads enables reaction clean-up, after ligation and PCR steps in general.
GB-Magic effectively removes salts, primers, primer-dimers and dNTPs. The protocol can be performed manually, or adapted to liquid handling workstations (e.g. Agilent, Beckman, Caliper, Eppendorf, Hamilton, PerkinElmer and Tecan) using individual tube, 96- or 384-well formats.
APPLICATIONS
GB-Magic is perfectly-suited to the following applications :
• NGS library preparation
• Sanger sequencing
• PCR
• Recovery from enzymatic reactions

GB-Lab™ Standard-Volume Vortex Mixer
1.Digital display available;Stepless speed reg µLation;Touch/ run easy to switch.

2. µLtra-low body stable operation/ Brushless motor with string power.

3.With various fixtures,suitable for centrif µgal tube, blood tube,PCR plate cell c µLtrue plate and so on.

4.You can mix whatever you want, such as tubes, plates of all Volumes.

5. Adjustable speed, 0 - 3000RPM.
6. Flat closed contour around the cup for the standard tube mod µLe for each cleaning
7. In addition to the standard mod µLe for Centrif µge tube/test tube, the following mod µLes are also available from us. Ask us now.
Flask or m µLtiple tubes
12mm diameter Capillary tubes*21 tubes
5ml/15ml Centrif µge tube*12 tubes
50ml Centrif µge tube*4 5ml/15ml*Centrif µge tube*5 tubes
"0.2ml Centrif µge tube*96 or 96 well plate"
"0.5ml Centrif µge tube*20 tubes
1.5ml/2.0ml Centrif µge tube *30 tubes
Cell c µLture plate

GB-Lab™ Mini Vortex Mixer
1.Smart and Portable / Tap to launch / Brushless motor / Stepless speed reg µLation

2.You can mix whatever you want in a tube or container, such as solutions, nail polishes, eyelash adhesives, paints, glues, tattoo ink, pigments and other to µgh liquids.

3.It is suitable for microcentrif µge tube, Eppendorf tube and 5 ml, 15ml and 50 ml tubes.
4. Adjustable speed, 0 - 3000RPM.

This enzyme is supplied in a buffer of 10 mM Tris-HCl (pH 7.5), 50 mM KCl, 10 mM 2-mercaptoethanol, 0.1 mM EDTA and 50% glycerol. It was purified from an E. coli strain expressing a recombinant clone of T4 DNA ligase. Its molec µLar Weight is 68kDa.
Requirements : Mg2+, ATP and DTT. The optimum concentration of Mg2+ is 10mM. Mn2+ may be
substituted for Mg2+ but is only 25% as effective as Mg2+.
Inhibition : 50% inhibition by greater than 150mM NaCl (activity measured at nicks. Other inhibitors
include 0.2M K+, Cs+, Li+, NH4+ and 1mM spermine.
Inactivation : Heat to 70°C for 10 minutes.
Contents : GB-Clone™ T4 DNA Ligase (200 U/μl)
10 X Ligation Buffer
We recommend that 0.5-1 μl of the ligase is used per 10 μl ligation reaction.

Applications
 Cloning of restriction fragments.
 Joining linkers and adapters to blunt-ended DNA
Unit Definition
One unit of GB-Clone™ T4 DNA Ligase is defined as the amount of enzyme required to give 50%
ligation of Hind III fragments of λ DNA (5´ DNA termini concentration of 0.12 μM, 300-
μg/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C in 1x T4 DNA Ligase
Reaction Buffer.

Storage： Store at -20°C
Storage Buffer : 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 200 μg/ml BSA and 50% glycerol.
10x T4 Ligase Buffer :
660mM Tris-HCl(PH7.6), 66mM MgCl2, 100mM DTT, 10mM ATP
Physical Purity
The purity is ≥90% as judged by SDS-polyacrylamide gel electrophoresis with
Coomassie® blue staining.
Standard Applications
We recommend using a 1 : 1 1 : 3 or 3 : 1 molar ratio of vector : insert DNA when cloning a
fragment into a plasmid vector. These ratios will vary with other types of vectors, for
example, cDNA and genomic cloning vectors. The following example illustrates the
conversion of molar ratios to mass ratios for a 3.0kb plasmid and a 0.5kb insert DNA
fragment.
Protocol :
The following ligation reaction of a 3kb vector and a 0.5kb insert DNA uses a 1 : 1
vector : insert ratio. Typical ligation reactions use 100–200ng of vector DNA.
1. Assemble the following reaction in a sterile microcentrif µge tube :
10 X Ligation Buffer 1 μl
vector DNA 100ng
insert DNA 1 : 1 to 10 : 1 molar ratio over vector
T4 DNA Ligase 0.5–1μl
Nuclease-Free Water to final volume of 10μl
2. Incubate the reaction at room temperature for 3 hours, or 16°C for 4-18 hours, or
4 °C over night.
Notes :
1. Concatamers may form as ligation products. The extent of concatamer formation
depends on the vector : insert ratio, incubation temperature and incubation time. This
sho µLd be taken into account when screening transformants.

GB-Clone™ FAST T4 DNA Ligase is designed for the efficient ligation of cohesive-ended DNA inserts
into plasmid vectors in just 5-10 minutes (blunt-ended inserts in as little as 15-30 minutes).
FAST T4 DNA Ligase catalyzes the joining of two strands of DNA between the
5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides in either a
cohesive-ended or blunt-ended configuration. The enzyme has also been shown to
catalyze the joining of RNA to either a DNA or RNA strand in a duplex molec µLe but will not
join single-stranded nucleic acids.
Biological Source : E. coli strain expressing a recombinant clone.
Molec µLar Weight : 68kDa.
Requirements : Mg2+, ATP and DTT. The optimum concentration of Mg2+ is 10mM. Mn2+ may be
substituted for Mg2+ but is only 25% as effective as Mg2+.
Inhibition : 50% inhibition by greater than 150mM NaCl (activity measured at nicks. Other inhibitors include 0.2M K+, Cs+, Li+, NH4+ and 1mM spermine.
Inactivation : Heat to 70°C for 10 minutes.
Contents : FAST T4 DNA Ligase (400 U/μl)
2 X Ligation Buffer
We recommend that 0.5-1 μl of the ligase is used per 10 μl ligation reaction.
Applications
 Cloning of restriction fragments.
 Joining linkers and adapters to blunt-ended DNA
Unit Definition
One unit of FAST T4 DNA Ligase is defined as the amount of enzyme required to give
50% ligation of Hind III fragments of λ DNA (5´ DNA termini concentration of 0.12 μM, 300-
μg/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C in 1x T4 DNA Ligase
Reaction Buffer.

Storage： Store at -20℃
Storage Buffer : 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 200 μg/ml BSA and 50% glycerol.
2X T4 DNA Ligase Buffer : 60mM Tris-HCl (pH 7.8), 20mM MgCl2, 20mM DTT, 2mM ATP and 10% PEG.
Physical Purity : The purity is ≥90% as judged by SDS-polyacrylamide gel electrophoresis with
Coomassie® blue staining.
Standard Applications
We recommend using a 1 : 1 1 : 3 or 3 : 1 molar ratio of vector : insert DNA when cloning a
fragment into a plasmid vector. These ratios will vary with other types of vectors, for
example, cDNA and genomic cloning vectors. The following example illustrates the
conversion of molar ratios to mass ratios for a 3.0kb plasmid and a 0.5kb insert DNA
fragment.
Protocol :
The following ligation reaction of a 3kb vector and a 0.5kb insert DNA uses a 1 : 1
vector : insert ratio. Typical ligation reactions use 100–200ng of vector DNA.
1. Assemble the following reaction in a sterile microcentrif µge tube :
vector DNA 100ng
insert DNA 17ng
2 X Ligation Buffer 5μl
T4 DNA Ligase 0.5–1μl
Nuclease-Free Water to final volume of 10μl
2. Incubate the reaction at room temperature for 5-10 minutes for cohesive-ended
ligations, or 15-30 minutes for blunt-ended ligations.
Notes :
1. Ligation reactions performed using the 2 X Ligation Buffer do not need to be cleaned up
before transformation.
2. Concatamers may form as ligation products. The extent of concatamer formation
depends on the vector : insert ratio, incubation temperature and incubation time. This
sho µLd be taken into account when screening transformants.

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Introduction
The GB-Clean™ Plasmid Miniprep Kit (HiPure Mini system) provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molec µLar biology laboratory applications. HiPure Plasmid Mini Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of tris buffer or water.
Details

Specifications
Features Specifications
Main Functions Isolation up to 35μg plasmid DNA from 1-5ml bacterial c µLture
Applications Enzyme digestion, sequencing, PCR, cloning, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrif µgation or vacuum)
Sample type Conventional plasmid, plasmid less than 30KB
Sample amount
High copy plasmid : 1-5ml c µLture medium
Low copy number plasmid : 5-10ml c µLture medium
Yield 5-35µg
Elution volume <span "="" style="outline : 0px; box-sizing : border-box; font-Volume : 16px;">≥30μl
Time per run Complete 1-24 samples in 30 minutes
Liquid carrying volume per column 800µl
Binding yield of column 35µg

Principle
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps : Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages
High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast - it takes only 1 minute to obtain supernatant by optimized solution (10 minutes for other brands)
High yield (see comparison below)- up to 35µg plasmid can be bound in one column

Kit Contents
Contents P100101 P100103
Purification Times 50 Preps 250 Preps
RNase A 2 mg 10 mg
Buffer P1 15 ml 80 ml
Buffer P2
15 ml
80 ml
Buffer P3
20 ml
100 ml
Buffer PW1
25 ml
140 ml
Buffer PW2* 10 ml 50 ml
Elution Buffer 6 ml 30 ml
HiPure DNA Mini Columns II 50 250
2 ml Collection Tubes 50 250

Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.

Experiment Data
See also the attached data figure.

Made from clear polystyrene with standard microplate footprint, the single well plates are ideal for screening libraries and DNA hybridization. Surface treated for cell c µLture or non-treated for bacterial growth and colony isolation.
Clear polystyrene is compatible with optical applications.
External dimensions : 128 x 86mm.
Tray can be frozen at -20°C.
8.4 cm² C µLtivated area; s µggested volume of medium : 35 mL.
Applications :
Immunofluorescence,
Scratching,
HE staining,
Cell transfection and more.
.

Also known as, 96 m µLtiwell plates, 96 well microplates, 96 well microtiter plates, 96 well plates, these plates are made in polypropylene. For some of the special applications, such as assay development, compound and sample storage etc., in which the more common polystyrene plates are not suitable.
These plates have 96 Round Wells, which U-bottom, with the volume of up to 0.4ml per well.
Non-sterile; polypropylene; 0.4 ml per well. No lid. Silicone matt or sealing films are available.

Other Volumes of this product are also available. Please inquire.
Description
GB-Amp™ Taq DNA Polymerase is purified from E. coli. expressing a cloned Thermus aquaticus DNA polymerase gene. This enzyme has a 5' → 3' DNA polymerase and a 5' → 3' exonuclease activity but lacks a 3' → 5' exonuclease activity. The enzyme consists of a single polypeptide with a molec µLar weight of approximately 94 kDa. Taq DNA polymerase is heat-stable.
Contents
Taq DNA Polymerase by defa µLt is packaged in 5 U/μl.
10×PCR Buffer with Mg++,

Applications
Standard PCR
DNA labeling
DNA sequencing
Numerous applications for which a high-quality, thermostable DNA polymerase is required

Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

Storage Buffer
20mM TrisCl ( pH8.0), 100mM KCl, 3.2mM MgCl2 1mM DTT，0.1% Triton X-100 ,0.1% Tween20, 0.2mg/ml BSA, 50% (v/v) glycerol

Note
-Recombinant Taq DNA Polymerase is the enzyme of choice for most PCR applications.
-The half-life of enzyme is >40 minutes at 95°C.
-The error rate of Taq DNA Polymerase in PCR is 2.2x10-5 errors per nt per cycle;

the accuracy (an inverse of the error rate) an average number of correct nucleotides incorporated before making an error, is 4.5x104.

- Taq DNA Polymerase accepts modified nucleotides (e.g. biotin-, digoxigenin-, fluorescent-labeled nucleotides) as substrates for the DNA synthesis.Compatible with TA cloning – generates PCR products with 3’-dA overhangs.
-Suitable for Taqman probe real time PCR
-Recommendations with Template DNA in a 50μl reaction volume
-recommended quantities of template per react
Store all components at –20°C

When using this product for qPCR reactions, reliable standard curve can be obtained in a wide range and accurate qualification of target genes can be achieved with high repeatability and reliability.
Content
Components
PCR-04-01
(125 rxn / 20 µl reaction)
PCR-04-02
(500 rxn / 20 µl reaction)
GB-Amp™ qPCR Probe Master Mix a
1.25 ml
1.25 ml × 4
50 X ROX Reference Dye 1 b
50 µl
200 μl
50 X ROX Reference Dye 2 b
50 µl
200 μl
a. Contains dNTP Mix, Mg2+, and our HotStart DNA Polymerase.
b. Used to rectify the error of fluorescence signals between different wells.

GB-Amp™ Power Green InFluor™ Green qPCR Mix
For research use only
Components
Component
P2101-01
P2101-02
P2101-03
2X Power Green qPCR Mix
1 ml
1 ml × 5
1 ml × 25
400 rxn/20μl reaction
2000 rxn/20μl reaction
10,000 rxn/20μl reaction
Storage
This reagent can be stored for 2 months at 4°C and protected from light. For longer storage, it sho µLd be kept at -20°C and protected from light.
Description
Power Green qPCR Mix is a ready-to-use, 2X concentrated mix that contains all the reagents (except template and primers) needed for the real-time qPCR in the Green I detection format. This product is compatible with most manufacturers' real-time fluorescent quantitative PCR instruments such as Applied Biosystems, Roche, Bio-Rad, Eppendorf, Corbett and so on.
The PowerGreen™ InFluor™ HotStart Taq DNA Polymerase in the mix is chemically modified, and it can reduce non-specific amplification more effectively than the antibody-modified polymerase. At the same time, the mix contains aptamer-based inhibitors, which further reduce the generation of the primer dimer and secondary metabolites. With this dual blocking mechanism, when the temperature below 45°C, the activity of the modified polymerase will be strongly suppressed, only when the temperature reached 72°C, it can be activated. This dual hot-start technique brings greater efficiency and specificity to PCR.
About InFluor™ Green-a nucleic acid (DNA and RNA) intercalating dye, which is f µLly compatible will all major real-time PCR (qPCR) systems, specifically, InFluor™ Green fluorescence can be read in the green channel of the real-time PCR thermal cyclers for quantitative PCR analyses. The InFluor™ does not inhibit PCR amplification. It supports melt curve analyses.
Applications
Gene expression analysis
Low-copy gene detection
Microarray validation
Gene knockdown validation
Features
Compatible with all major real-time PCR systems, which accept No ROX (not requiring ROX reference dye), Low ROX or High ROX
Exceptional specificity with dual hot-start mechanism
Tight reproducibility in Ct values over a broad dynamic range
Universal instrument compatibility
Table of Instrument Guide
Instruments
ROX
Cat. #
ABI PRISM 7000/ PRISM 7700/ 7300/ 7900HT/ StepOne/ StepOnePlus/ GeneAmp 5700
High ROX
P2101-01
P2101-02
P2101-03
ABI 7500/ 7500 Fast/ ViiA 7/ QuantStudio 6/7/12K Flex; Agilent Stratagene Mx3000P/ Mx3005P/ Mx4000
Low ROX
P2101-01a
P2101-02a
P2101-03a
Bio-Rad CFX96/ CFX384/ iQ/ iQ5; MJ Research Opticon 2/ Chromo 4; Roche LightCycler 480/ 96; Corbett Rotor Gene G/ Q/ 3000/ 6000; Thermo PikoReal 96; Eppendorf MasterCycler ep realplex; Cepheid Smart Cycler
No ROX
P2101-01b
P2101-02b
P2101-03b

GB-AMP™ PaCeR™ HP™ DNA polymerase was derived from Pfu DNA Polymerase, after several iterative rounds of protein engineering, the enzyme boasts the on-board unique extension factor, specificity-promoting factors and plateau-inhibiting factor. It is a Fast Pfu derived high performance polymerase.
For a brochure on this product, please follow this link.
-Super Fidelity : 53-fold higher than Taq DNA Polymerase and 6-fold better than Pfu DNA polymerase
-Long Range capability : amplify fragments as long as 40 kbλ DNA, 20 kb genomic DNA and 10 kb cDNA
-Diffic µLt PCR suitability : Amplify targets with as high as 85% GC
-Direct-PCR : resists inhibitors in crude lysates of bacteria, fungi, whole blood, c µLtured cells, plant tissues, etc.
-Superior Hot-Start enzyme, employing two MAb as molec µLar thermal switches of polymerase and proof-reading activities.
-Fast PCR : rate of synthesis more than 2x that of Taq DNA polymerase
In an internal comparison studies, GB-AMP™ PaCeR™ HP™ was compared in the following aspects with the leading all-round enzymes such as Phusion (Thermo) and eight other leading DNA polymerases for PCR :
a) Success rate in amplifying human, mouse, rice, wheat, lambda and plasmid DNA, ranging in Volume from 305 to 14768 bp
b) Sensitivity, i.e., ability to detect low copy templates,
c) Speed of amplification with extension time as short as 1 s (for 450 bp) and 5 s (1135 bp)
d) Amplification of High GC template with GC as high as 85%
GB-AMP™ PaCeR™ HP™was clearly the best all-round enzyme for robustness, sensitivity, speed, high GC amplification and Direct PCR!
The enzyme generates blunt-ended products and thus products can be cloned by our Blunt End Cloning Kits.

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or
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GB-Amp™ InFluor™ Green qPCR Mix is a Taq DNA polymerase-based 2 x master mix for real-time PCR, which contains all components, except for the primer. This reagent is applicable for intercalation assay with InFluor™ Green.
About InFluor™ Green-a nucleic acid (DNA and RNA) intercalating dye, which is f µLly compatible will all major real-time PCR (qPCR) systems, specifically, InFluor™ Green fluorescence can be read in the green channel of the real-time PCR thermal cyclers for quantitative PCR analyses. The InFluor™ does not inhibit PCR amplification. It supports melt curve analyses.


Applications
• Real-Time PCR
• Real-Time PCR RT-PCR
The system contains sufficient reagents suitable for 200 amplification reactions of 50 μl PCR system. Nuclease-Free Water is also provided for your convenience in setting up PCR reactions.

Storage
This reagent can be stored at 4°C for 2 months when stored protected from light. For longer storage, this reagent sho µLd be kept at -20°C and protected from light.

Features
• This reagent can be used in glass capillary systems (e.g., LightCycler, Roche Molec µLar Systems, Inc.).
• This reagent can be used in a passive reference system (e.g., ABI PRISM® 7700, Applied Biosystems, Inc.). The passive reference dye does not affect any other systems.
• Hot Start technology with anti-Taq DNA polymerase antibodies enables high specificity and reproducible amplification.

Composition of our qPCR Mix (when diluted with your primers and samples)
100 mM KCl , 4 mM MgCl2, 400 μM dNTPs, 0.1 U/μl Taq DNA Polymerase, 1x InFluor™ Green and other optimized buffer components.

Detection
• This reagent can be used in general detection devices, such as : LineGene (Bioer Technology co., ltd.)
• This reagent can also be used in detection equipment using glass capillaries or passive reference, such as :
LightCycler (Roche Molec µLar Systems)
ABI PRISM® 7000, 7700, and 7900 (Applied Biosystems)
Note : The passive reference mode of detectors sho µLd be set at“ROX”.

Guidelines for preventing contamination of PCR reaction
During PCR more than 10 million copies of template DNA are generated. Therefore, care must be taken to avoid contamination with other templates and amplicons that may be present in the laboratory environment. General recommendations to lower the risk of contamination are as follows :
• Prepare your DNA sample, set up the PCR mixture, perform thermal cycling and analyze PCR products in separate areas.
• Set up PCR mixtures in a laminar flow cabinet equipped with an UV lamp.
• Wear fresh gloves for DNA purification and reaction set up.
• Use reagent containers dedicated for PCR. Use positive displacement pipettes, or use pipette tips with aerosol filters to prepare DNA samples and perform PCR set up.
• Always perform “no template control” (NTC) reactions to check for contamination
Quality Control
The absence of endodeoxyribonucleases, exodeoxyribonucl- eases and ribonucleases is confirmed by appropriate quality tests. Functionally tested in amplification of a single-copy gene from human genomic DNA.

Description
GB-Amp™ HotStart Taq DNA polymerase is a hot-start polymerase with chemical modification, which brings higher specificity by reducing non-specific products as the enzyme activity is temperature-dependent and is inhibited at room temperature. The amplification length and speed can reach to 5 kb (simple template) and 0.5 kb/min separately. Hotstart Taq has 5’-3’ polymerase activity, but no 3’-5’ exonuclease activity. The products of Taq plus have overhanged dA at 3’-end.
Components : Hotstart Taq DNA polymerase (5U/ µL); 10 Hotstart Buffer (including Mg), 6 x gel loading buffer
Stable for 2 years at -20°C
GB-Amp™ HotStart Taq DNA polymerase has zero animal source pollution by being produced with advanced chemical modification. And it is much more stable than antibody-modified hot-start polymerase. Its efficiency is higher than most chemical-modified polymerase and the initial-denaturation time can be reduced to 3 minutes.
Unit Definition
One enzyme unit (U) refers to the amount of enzyme needed for intaking 10 nmol nucleotides when using activated salmon sperm DNA as template/primer, at 72 ℃, in 30 minutes
Quality control
The absence of endonuclease or exonuclease is confirmed by appropriate quality tests. PCR detects no host residual DNA, and it can effectively amplify the single-copy gene of human genome. There is no significant change about the amplification activity after one week at room temperature.
Storage Buffer
200 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Tween 20, 50% Glycerol, 0.5% Triton X -100
Notes
1 This product adopts improved chemical modification technology, so it relies on temperature to activate the polymerase activity, which can effectively inhibit non-specific bindings, and the reaction system can be configured at room temperature.
2 This Hotstart Taq DNA polymerase has the deoxidizing nucleotide transfer activity, so at the 3’-end of the PCR product is usually added to an extra deoxygenated adenosine nucleoside.
Endodeoxyribonuclease Assay
No detectable conversion of covalently closed circ µLar DNA to a nicked DNA was observed after incubation of 10U GB-Amp™ HotStart Taq DNA polymerase with 1μg pBR322 DNA for 4 hours at 37°C and 70°C.
Exodeoxyribonuclease Assay
No detectable degradation of lambda DNA-HindIII fragments was observed after incubation of 10U GB-Amp™ HotStart Taq DNA polymerase with 1μg digested DNA for 4 hours at 37°C and 70°C.
Ribonuclease Assay
0% of the total radioactivity was released into trichloroacetic acid-soluble fraction after incubation of 10U GB-Amp™ HotStart Taq DNA polymerase with 1μg E.coli [3H]-RNA (40000cpm/μg) for 4 hours at 37°C and 70°C.
Q&A
Why the specificity of GB-Amp™ HotStart Taq DNA polymerasee is higher than ordinary Taq DNA polymerase?
The Hotstart Taq DNA polymerase is a chemically modified Taq DNA polymerase, which activity is completely inhibited at room temperature and it is activated at high temperature in a very short time. Low temperature inhibition & high temperature activation keep the non-specific reactions, i.e., primer dimer formation and off-target amplification low, and thus the specificity high.
Are the products of Hotstart Taq DNA polymerase compatible to TA cloning?
Yes. Because the products have 3’-dA protruding ends.

Used for end-point PCR, this product is available in a version containing electrophoresis buffer and dye. It can be directly electrophoresed after the reaction. The 3' end of the PCR product, A base, can be directly cloned into the T vectors (e.g., our TA-cloning kits : https : //www.genebiosystems.com/50-ta-cloning-kits)
When using this product for qPCR reactions, reliable standard curve can be obtained in a wide range and accurate qualification of target genes can be achieved with high repeatability and reliability.
This master mix contains loading dye for gel, so that after PCR, you can just load the PCR product to gel, without having to add gel loading dye.

Used for end-point PCR, this product is available in a version containing electrophoresis buffer and dye. It can be directly electrophoresed after the reaction. The 3' end of the PCR product, A base, can be directly cloned into the T vectors (e.g., our TA-cloning kits : https : //www.genebiosystems.com/50-ta-cloning-kits)
When using this product for qPCR reactions, reliable standard curve can be obtained in a wide range and accurate qualification of target genes can be achieved with high repeatability and reliability.
Please note : this product does not contain loading dye for gel electrophoresis, therefore, loading buffer with dye must be added before loading PCR products onto gels.
For the HotStart Taq 2 x Master Mix pre-spiked with loading dye, please refer to GB-Amp™ HotStart Taq 2x Master Mix (with Loading Dye)
Cat. # :
P2041b https : //www.genebiosystems.com/pcr-reagents/260802-hot-start-taq-2x-master-mix.html

10% OFF list price for the 50ml variant as part of our FALL Sale! On now until October 18th, 2024!
Cryoprotective reagents in the 2× Taq Master Mix enable the resistance to repeated freeze-thaw cycles. With more than 13 high-impact factor journals citing the use of this product and known for its robust amplification for up to 6 kb of PCR products in Volume and resistance to impurities, this reagent sho µLd work for most of your PCR and amplification applications*.
GB-Amp™ 2 x PCR Master mix (without loading dye) is provided without gel loading dyes. If you need a robust 2x PCR Master Mix with an insert gel loading dye already added, please consider our BLU-Taq™ 2× Taq Master Mix.
Taq DNA Polymerase included in this master mix is a thermostable DNA polymerase that possesses a 5 '→3 ' polymerase activity and a 5 ' flap endonuclease activity. The PCR products contain A at the 3'-end and thus can be directly cloned into T-Vectors of TA cloning kits.
GB-Amp™ 2 x PCR Master mix (without loading dye)
Cat#
Volume
Price
List Price ($)/ml
GB-Amp™ 2 x PCR Master mix (without loading dye)
P4001
1 mL
26
26
P4002
5 mL
85
17
P4003
10 mL
140
14
P4004
50 mL
390
7.8

10% OFF list price for the 50ml variant as part of our FALL Sale! On now until October 18th, 2024!
PCR Master Mix is a premixed solution containing all essential components and reagents required to conduct a PCR assay. It significantly simplifies the analysis of PCR products and the preparation of PCR samples. It is specified for requiring high sensitivity, such as somatic mutations and microbial DNA detection. Generally, the master mix catalog includes thermostable Taq DNA polymerase recombinant, dNTPs, stabilizers, cofactor (MgCl2), and enhancers, all in a reaction buffer.
APPLICATIONS OF GB-AMP™ 2X PCR MASTER MIX PRODUCTS
Routine PCR
Primer dimers reduction
Various primer-extension reactions, including labeling, signaling, and sequencing

FEATURES AND BENEFITS OF OUR PRODUCT
Incredible amplification — The Green PCR Master guarantees up to 6 kb amplification of PCR products.
It is resistant to impurities.
Quick and convenient — Our 2x master mixes are equipped with gel loading dyes and other additives, including Taq DNA polymers facilitating direct loading of the products and templates onto the agarose gels.
The GB-Amp™ 2X universal PCR Master Mix is designed to make work easier, save time, and eliminate errors in proportioning and measurements. If you have been looking to streamline and improve your lab experiments, the state-of-the-art GB-Amp™ 2X Universal PCR Master Mix is a great option.
Do you need to use both Taq, Pfu or any other proofreading polymerase for PCR? In this case, our 5min TA-Blunt-Zero Cloning Kit is the perfect choice. This is where we come in. We have your back, no matter the scope and Volume of your laboratory.

A mixture of dNTPs with 25 mM each of dATP, dCTP, dGTP, dTTP. RNAse, DNAse-free, high purity. >2 year Stablity at -20 C. Can be used in polymerase and other enzyme catalyzed reactions, such as PCR, isothermal amplification and sequencing reactions.

A mixture of dNTPs with 10mM each of dATP, dCTP, dGTP, dTTP. RNAse, DNAse-free, high purity. >2 year Stablity at -20 C. Can be used in polymerase and other enzyme catalyzed reactions, such as PCR, isothermal amplification and sequencing reactions.

Can be used in polymerase and other enzyme catalyzed reactions, such as PCR, isothermal amplification and sequencing reactions.

Can be used in polymerase and other enzyme catalyzed reactions, such as PCR, isothermal amplification and sequencing reactions. Available in two Volumes, 4x0.25 ml and 4 x 1 ml.

Volume : 50 µg
Clone Number :
Aliases : gB antibody; µL27 antibody; Envelope glycoprotein B antibody; gB antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human) herpesvirus 2 (strain HG52) (HHV-2) (Homo sapiens (Human) herpes simplex virus 2)
UniProt ID : P08666
Immunogen : Recombinant Human herpesvirus 2 Envelope glycoprotein B protein (23-279AA)
Raised in : Rabbit
Species Reactivity : Human herpesvirus 2
Tested Applications : ELISA
Background : Envelope glycoprotein that forms spikes at the surface of virion envelope. Essential for the initial attachment to heparan s µLfate moities of the host cell surface proteoglycans. Involved in fusion of viral and cell µLar membranes leading to virus entry into the host cell. Following initial binding to its host receptors, membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. May be involved in the fusion between the virion envelope and the outer nuclear membrane during virion egress.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : gB
Research Areas : Others

Volume : 50 µg
Clone Number :
Aliases : gB antibody; µL27 antibody; Envelope glycoprotein B antibody; gB antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human) herpesvirus 2 (strain HG52) (HHV-2) (Homo sapiens (Human) herpes simplex virus 2)
UniProt ID : P08666
Immunogen : Recombinant Human herpesvirus 2 Envelope glycoprotein B protein (23-279AA)
Raised in : Rabbit
Species Reactivity : Human herpesvirus 2
Tested Applications :
Background : Envelope glycoprotein that forms spikes at the surface of virion envelope. Essential for the initial attachment to heparan s µLfate moities of the host cell surface proteoglycans. Involved in fusion of viral and cell µLar membranes leading to virus entry into the host cell. Following initial binding to its host receptors, membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. May be involved in the fusion between the virion envelope and the outer nuclear membrane during virion egress.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : gB
Research Areas : Others

Volume : 50 µg
Clone Number :
Aliases : gB antibody; µL27 antibody; Envelope glycoprotein B antibody; gB antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human) herpesvirus 2 (strain HG52) (HHV-2) (Homo sapiens (Human) herpes simplex virus 2)
UniProt ID : P08666
Immunogen : Recombinant Human herpesvirus 2 Envelope glycoprotein B protein (23-279AA)
Raised in : Rabbit
Species Reactivity : Human herpesvirus 2
Tested Applications : ELISA
Background : Envelope glycoprotein that forms spikes at the surface of virion envelope. Essential for the initial attachment to heparan s µLfate moities of the host cell surface proteoglycans. Involved in fusion of viral and cell µLar membranes leading to virus entry into the host cell. Following initial binding to its host receptors, membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. May be involved in the fusion between the virion envelope and the outer nuclear membrane during virion egress.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Biotin
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : gB
Research Areas : Others

Volume : 50 µg
Clone Number :
Aliases : gB antibody; µL27 antibody; Envelope glycoprotein B antibody; gB antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human) herpesvirus 2 (strain HG52) (HHV-2) (Homo sapiens (Human) herpes simplex virus 2)
UniProt ID : P08666
Immunogen : Recombinant Human herpesvirus 2 Envelope glycoprotein B protein (23-279AA)
Raised in : Rabbit
Species Reactivity : Human herpesvirus 2
Tested Applications : ELISA
Background : Envelope glycoprotein that forms spikes at the surface of virion envelope. Essential for the initial attachment to heparan s µLfate moities of the host cell surface proteoglycans. Involved in fusion of viral and cell µLar membranes leading to virus entry into the host cell. Following initial binding to its host receptors, membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. May be involved in the fusion between the virion envelope and the outer nuclear membrane during virion egress.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Non-conj µgated
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : gB
Research Areas : Others

Volume : 100 µg
Purification : Immunogen affinity purified
Form : liquid
Purity : 95% as determined by SDS-PAGE
Host : Rabbit
Clonality : polyclonal Ab
Clone ID :
Isotype : IgG
Storage : PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20? for 12 months (Avoid repeated freeze / thaw cycles.)
Background : This gene encodes a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis, whereby it catalyzes the transfer of a guanido group from L-arginine to glycine, res µLting in guanidinoacetic acid, the immediate precursor of creatine. Mutations in this gene cause arginine : glycine amidinotransferase deficiency, an inborn error of creatine synthesis characterized by mental retardation, language impairment, and behavioral disorders.
Immunogen : glycine amidinotransferase (L-arginine : glycine amidinotransferase)
Aliases : AGAT
Observed MW : 43 kDa
Uniprot ID : P50440
Reactivity : Human, Mouse, Rat
Application : ELISA, WB, IHC, IF
Recommended dilution : WB : 1 : 500 - 1 : 2000; IHC : 1 : 50 - 1 : 200; IF : 1 : 50 - 1 : 200
Gene ID : 2628
Research Area : Metabolism

Volume : 50 µg
Clone Number :
Aliases : GATD3A antibody; C21orf33 antibody; Glutamine amidotransferase-like class 1 domain-containing protein 3A antibody; mitochondrial antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P0DPI2
Immunogen : Recombinant Human Glutamine amidotransferase-like class 1 domain-containing protein 3A, mitochondrial protein (43-268AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background :
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATD3A
Research Areas : Tags & Cell Markers; Metabolism

Volume : 50 µg
Clone Number :
Aliases : GATD3A antibody; C21orf33 antibody; Glutamine amidotransferase-like class 1 domain-containing protein 3A antibody; mitochondrial antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P0DPI2
Immunogen : Recombinant Human Glutamine amidotransferase-like class 1 domain-containing protein 3A, mitochondrial protein (43-268AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background :
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATD3A
Research Areas : Tags & Cell Markers; Metabolism

Volume : 50 µg
Clone Number :
Aliases : GATD3A antibody; C21orf33 antibody; Glutamine amidotransferase-like class 1 domain-containing protein 3A antibody; mitochondrial antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P0DPI2
Immunogen : Recombinant Human Glutamine amidotransferase-like class 1 domain-containing protein 3A, mitochondrial protein (43-268AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background :
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Biotin
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATD3A
Research Areas : Tags & Cell Markers; Metabolism

Volume : 50 µg
Clone Number :
Aliases : GATD3A antibody; C21orf33 antibody; Glutamine amidotransferase-like class 1 domain-containing protein 3A antibody; mitochondrial antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P0DPI2
Immunogen : Recombinant Human Glutamine amidotransferase-like class 1 domain-containing protein 3A, mitochondrial protein (43-268AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA, WB, IHC; Recommended dilution : WB : 1 : 500-1 : 5000, IHC : 1 : 20-1 : 200
Background :
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Non-conj µgated
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATD3A
Research Areas : Tags & Cell Markers; Metabolism

Volume : 100 µg
Purification : Immunogen affinity purified
Form : liquid
Purity : 95% as determined by SDS-PAGE
Host : Rabbit
Clonality : polyclonal Ab
Clone ID :
Isotype : IgG
Storage : PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.)
Background : Allows the formation of correctly charged Gln-tRNA(Gln) thro µgh the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP thro µgh an activated gamma-phospho-Glu-tRNA(Gln).
Immunogen : glutamyl-tRNA(Gln) amidotransferase, subunit C homolog(bacterial)
Aliases : 15E1.2
Observed MW : 15 kDa
Uniprot ID : O43716
Reactivity : Human, Mouse
Application : ELISA, IHC, IP
Recommended dilution : IP : 1 : 200-1 : 2000; IHC : 1 : 20-1 : 200
Gene ID : 283459
Research Area : Metabolism

Volume : 50 µg
Clone Number :
Aliases : GATB antibody; PET112 antibody; PET112L antibody; HSPC199Glutamyl-tRNA(Gln) amidotransferase subunit B antibody; mitochondrial antibody; Glu-AdT subunit B antibody; EC 6.3.5.- antibody; Cytochrome c oxidase assembly factor PET112 homolog antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : O75879
Immunogen : Recombinant Human Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial protein (301-383AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : Allows the formation of correctly charged Gln-tRNA(Gln) thro µgh the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP thro µgh an activated gamma-phospho-Glu-tRNA(Gln).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATB
Research Areas : Epigenetics and Nuclear Signaling; Tags & Cell Markers; Metabolism

Volume : 50 µg
Clone Number :
Aliases : GATB antibody; PET112 antibody; PET112L antibody; HSPC199Glutamyl-tRNA(Gln) amidotransferase subunit B antibody; mitochondrial antibody; Glu-AdT subunit B antibody; EC 6.3.5.- antibody; Cytochrome c oxidase assembly factor PET112 homolog antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : O75879
Immunogen : Recombinant Human Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial protein (301-383AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background : Allows the formation of correctly charged Gln-tRNA(Gln) thro µgh the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP thro µgh an activated gamma-phospho-Glu-tRNA(Gln).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATB
Research Areas : Epigenetics and Nuclear Signaling; Tags & Cell Markers; Metabolism

Volume : 50 µg
Clone Number :
Aliases : GATB antibody; PET112 antibody; PET112L antibody; HSPC199Glutamyl-tRNA(Gln) amidotransferase subunit B antibody; mitochondrial antibody; Glu-AdT subunit B antibody; EC 6.3.5.- antibody; Cytochrome c oxidase assembly factor PET112 homolog antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : O75879
Immunogen : Recombinant Human Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial protein (301-383AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : Allows the formation of correctly charged Gln-tRNA(Gln) thro µgh the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP thro µgh an activated gamma-phospho-Glu-tRNA(Gln).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Biotin
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATB
Research Areas : Epigenetics and Nuclear Signaling; Tags & Cell Markers; Metabolism

Volume : 50 µg
Clone Number :
Aliases : GATB antibody; PET112 antibody; PET112L antibody; HSPC199Glutamyl-tRNA(Gln) amidotransferase subunit B antibody; mitochondrial antibody; Glu-AdT subunit B antibody; EC 6.3.5.- antibody; Cytochrome c oxidase assembly factor PET112 homolog antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : O75879
Immunogen : Recombinant Human Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial protein (301-383AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA, WB, IHC, IF; Recommended dilution : WB : 1 : 500-1 : 5000, IHC : 1 : 200-1 : 500, IF : 1 : 50-1 : 200
Background : Allows the formation of correctly charged Gln-tRNA(Gln) thro µgh the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP thro µgh an activated gamma-phospho-Glu-tRNA(Gln).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Non-conj µgated
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATB
Research Areas : Epigenetics and Nuclear Signaling; Tags & Cell Markers; Metabolism

Volume : 50 µg
Clone Number :
Aliases : FLJ37346 antibody; GATA zinc finger domain containing 2B antibody; GATA zinc finger domain containing protein 2B antibody; GATA zinc finger domain-containing protein 2B antibody; GATAD2B antibody; KIAA1150 antibody; MGC138257 antibody; MGC138285 antibody; mKIAA1150 antibody; p66/p68 antibody; P66B_HUMAN antibody; P66beta antibody; RGD1308533 antibody; transcription repressor p66 beta component of the MeCP1 complex antibody; Transcriptional repressor p66 beta antibody; Transcriptional repressor p66-beta antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q8WXI9
Immunogen : Recombinant Human Transcriptional repressor p66-beta protein (58-146AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : Transcriptional repressor. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2A. Targets MBD3 to discrete loci in the nucleus. May play a role in synapse development.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATAD2B
Research Areas : Epigenetics and Nuclear Signaling

Volume : 50 µg
Clone Number :
Aliases : FLJ37346 antibody; GATA zinc finger domain containing 2B antibody; GATA zinc finger domain containing protein 2B antibody; GATA zinc finger domain-containing protein 2B antibody; GATAD2B antibody; KIAA1150 antibody; MGC138257 antibody; MGC138285 antibody; mKIAA1150 antibody; p66/p68 antibody; P66B_HUMAN antibody; P66beta antibody; RGD1308533 antibody; transcription repressor p66 beta component of the MeCP1 complex antibody; Transcriptional repressor p66 beta antibody; Transcriptional repressor p66-beta antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q8WXI9
Immunogen : Recombinant Human Transcriptional repressor p66-beta protein (58-146AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background : Transcriptional repressor. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2A. Targets MBD3 to discrete loci in the nucleus. May play a role in synapse development.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATAD2B
Research Areas : Epigenetics and Nuclear Signaling

Volume : 50 µg
Clone Number :
Aliases : FLJ37346 antibody; GATA zinc finger domain containing 2B antibody; GATA zinc finger domain containing protein 2B antibody; GATA zinc finger domain-containing protein 2B antibody; GATAD2B antibody; KIAA1150 antibody; MGC138257 antibody; MGC138285 antibody; mKIAA1150 antibody; p66/p68 antibody; P66B_HUMAN antibody; P66beta antibody; RGD1308533 antibody; transcription repressor p66 beta component of the MeCP1 complex antibody; Transcriptional repressor p66 beta antibody; Transcriptional repressor p66-beta antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q8WXI9
Immunogen : Recombinant Human Transcriptional repressor p66-beta protein (58-146AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : Transcriptional repressor. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2A. Targets MBD3 to discrete loci in the nucleus. May play a role in synapse development.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Biotin
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATAD2B
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µg
Purification : Immunogen affinity purified
Form : liquid
Purity : 95% as determined by SDS-PAGE
Host : Rabbit
Clonality : polyclonal Ab
Clone ID :
Isotype : IgG
Storage : PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.)
Background : Transcriptional repressor. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2A. Targets MBD3 to discrete loci in the nucleus. May play a role in synapse development.
Immunogen : GATA zinc finger domain containing 2B
Aliases : KIAA1150
Observed MW : 69 kDa
Uniprot ID : Q8WXI9
Reactivity : Human
Application : ELISA, WB, IF
Recommended dilution : WB : 1 : 500-1 : 2000; IF : 1 : 20-1 : 200
Gene ID : 57459
Research Area : Metabolism

Volume : 50 µg
Clone Number :
Aliases : FLJ20085 antibody; FLJ21017 antibody; GATA zinc finger domain containing 2A antibody; GATA zinc finger domain containing protein 2A antibody; GATA zinc finger domain-containing protein 2A antibody; GATAD2A antibody; Hp66 alpha antibody; Hp66alpha antibody; OTTHUMP00000201911 antibody; OTTHUMP00000201912 antibody; P66 alpha antibody; P66A_HUMAN antibody; p66alpha antibody; Transcriptional repressor p66 alpha antibody; Transcriptional repressor p66-alpha antibody; Transcriptional repressor p66alpha antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q86YP4
Immunogen : Recombinant Human Transcriptional repressor p66-alpha protein (170-241AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : Transcriptional repressor. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2B.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATAD2A
Research Areas : Epigenetics and Nuclear Signaling

Volume : 50 µg
Clone Number :
Aliases : FLJ20085 antibody; FLJ21017 antibody; GATA zinc finger domain containing 2A antibody; GATA zinc finger domain containing protein 2A antibody; GATA zinc finger domain-containing protein 2A antibody; GATAD2A antibody; Hp66 alpha antibody; Hp66alpha antibody; OTTHUMP00000201911 antibody; OTTHUMP00000201912 antibody; P66 alpha antibody; P66A_HUMAN antibody; p66alpha antibody; Transcriptional repressor p66 alpha antibody; Transcriptional repressor p66-alpha antibody; Transcriptional repressor p66alpha antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q86YP4
Immunogen : Recombinant Human Transcriptional repressor p66-alpha protein (170-241AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background : Transcriptional repressor. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2B.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATAD2A
Research Areas : Epigenetics and Nuclear Signaling

Volume : 50 µg
Clone Number :
Aliases : FLJ20085 antibody; FLJ21017 antibody; GATA zinc finger domain containing 2A antibody; GATA zinc finger domain containing protein 2A antibody; GATA zinc finger domain-containing protein 2A antibody; GATAD2A antibody; Hp66 alpha antibody; Hp66alpha antibody; OTTHUMP00000201911 antibody; OTTHUMP00000201912 antibody; P66 alpha antibody; P66A_HUMAN antibody; p66alpha antibody; Transcriptional repressor p66 alpha antibody; Transcriptional repressor p66-alpha antibody; Transcriptional repressor p66alpha antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q86YP4
Immunogen : Recombinant Human Transcriptional repressor p66-alpha protein (170-241AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : Transcriptional repressor. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2B.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Biotin
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATAD2A
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : GATAD2A
GeneID : 54815
Uniprot : Q86YP4
Specificity : GATAD2A Antibody detects endogenous levels of GATAD2A.
Immunogen : A syntheVolumed peptide derived from human GATAD2A.
Storage : Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Reactivity : Human,Mouse
Prediction :
Application : ELISA(peptide)
Dilution : ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID :
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : DF14789-BP
Des# Blocking Peptide : GATAD2A Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 100 µL
Clone Number :
Aliases : ASD9, AVSD5, PACHD
Product Type : Monoclonal Antibody
Immunogen Species : Human
UniProt ID : Q92908
Immunogen : Purified recombinant human GATA6 protein fragments expressed in E.coli.
Raised in : Mouse
Species Reactivity : Human
Tested Applications : ELISA,WB;Recommended dilution : WB : 1 : 500-1 : 5000
Background : Tho µght to be important for reg µLating terminal differentiation and/or proliferation.
Clonality : Monoclonal
Isotype : IgG
Purification Method : Affinity purified
Conj µgate : Non-conj µgated
Buffer : Ascites
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATA6
Research Areas : Epigenetics and Nuclear Signaling;Cancer?Cardiovasc µLar;Developmental biology;Stem cells

Volume : 50 µg
Clone Number :
Aliases : Gata binding factor 6 antibody; Gata binding protein 6 antibody; GATA-binding factor 6 antibody; Gata6 antibody; GATA6_HUMAN antibody; Transcription factor Gata 6 antibody; Transcription factor GATA-6 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q92908
Immunogen : Recombinant Human Transcription factor GATA-6 protein (496-595AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : Transcriptional activator that reg µLates SEMA3C and PLXNA2 (PubMed : 19666519). Involved in gene reg µLation specifically in the gastric epithelium (PubMed : 9315713). May reg µLate genes that protect epithelial cells from bacterial infection (PubMed : 16968778). Involved in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATA6
Research Areas : Epigenetics and Nuclear Signaling; Cancer; Cardiovasc µLar; Developmental biology; Stem cells

Volume : 50 µg
Clone Number :
Aliases : Gata binding factor 6 antibody; Gata binding protein 6 antibody; GATA-binding factor 6 antibody; Gata6 antibody; GATA6_HUMAN antibody; Transcription factor Gata 6 antibody; Transcription factor GATA-6 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q92908
Immunogen : Recombinant Human Transcription factor GATA-6 protein (496-595AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background : Transcriptional activator that reg µLates SEMA3C and PLXNA2 (PubMed : 19666519). Involved in gene reg µLation specifically in the gastric epithelium (PubMed : 9315713). May reg µLate genes that protect epithelial cells from bacterial infection (PubMed : 16968778). Involved in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATA6
Research Areas : Epigenetics and Nuclear Signaling; Cancer; Cardiovasc µLar; Developmental biology; Stem cells

Volume : 50 µg
Clone Number :
Aliases : Gata binding factor 6 antibody; Gata binding protein 6 antibody; GATA-binding factor 6 antibody; Gata6 antibody; GATA6_HUMAN antibody; Transcription factor Gata 6 antibody; Transcription factor GATA-6 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q92908
Immunogen : Recombinant Human Transcription factor GATA-6 protein (496-595AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : Transcriptional activator that reg µLates SEMA3C and PLXNA2 (PubMed : 19666519). Involved in gene reg µLation specifically in the gastric epithelium (PubMed : 9315713). May reg µLate genes that protect epithelial cells from bacterial infection (PubMed : 16968778). Involved in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Biotin
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATA6
Research Areas : Epigenetics and Nuclear Signaling; Cancer; Cardiovasc µLar; Developmental biology; Stem cells

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : GATA6
GeneID : 2627
Uniprot : Q92908
Specificity : GATA6 Antibody detects endogenous levels of total GATA6.
Immunogen : A syntheVolumed peptide derived from human GATA6, corresponding to a region within the internal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse,Rat
Prediction : Pig,Zebrafish,Bovine,Sheep,Chicken,Xenopus
Application : WB,IF/ICC,ELISA(peptide)
Dilution : WB 1 : 500-1 : 2000, IF/ICC 1 : 100-1 : 500, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2837756
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : AF5270-BP
Des# Blocking Peptide : GATA6 Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 50 µg
Clone Number :
Aliases : GATA binding protein 5 antibody; GATA-binding factor 5 antibody; GATA5 antibody; GATA5_HUMAN antibody; Transcription factor GATA-5 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q9BWX5
Immunogen : Recombinant Human Transcription factor GATA-5 protein (336-397AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : Transcription factor required during cardiovasc µLar development (PubMed : 23289003). Plays an important role in the transcriptional program(s) that underlies smooth muscle cell diversity (By similarity). Binds to the functionally important CEF-1 nuclear protein binding site in the cardiac-specific slow/cardiac troponin C transcriptional enhancer (PubMed : 25543888).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATA5
Research Areas : Epigenetics and Nuclear Signaling

Volume : 50 µg
Clone Number :
Aliases : GATA binding protein 5 antibody; GATA-binding factor 5 antibody; GATA5 antibody; GATA5_HUMAN antibody; Transcription factor GATA-5 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q9BWX5
Immunogen : Recombinant Human Transcription factor GATA-5 protein (336-397AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background : Transcription factor required during cardiovasc µLar development (PubMed : 23289003). Plays an important role in the transcriptional program(s) that underlies smooth muscle cell diversity (By similarity). Binds to the functionally important CEF-1 nuclear protein binding site in the cardiac-specific slow/cardiac troponin C transcriptional enhancer (PubMed : 25543888).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATA5
Research Areas : Epigenetics and Nuclear Signaling

Volume : 50 µg
Clone Number :
Aliases : GATA binding protein 5 antibody; GATA-binding factor 5 antibody; GATA5 antibody; GATA5_HUMAN antibody; Transcription factor GATA-5 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q9BWX5
Immunogen : Recombinant Human Transcription factor GATA-5 protein (336-397AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : Transcription factor required during cardiovasc µLar development (PubMed : 23289003). Plays an important role in the transcriptional program(s) that underlies smooth muscle cell diversity (By similarity). Binds to the functionally important CEF-1 nuclear protein binding site in the cardiac-specific slow/cardiac troponin C transcriptional enhancer (PubMed : 25543888).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Biotin
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATA5
Research Areas : Epigenetics and Nuclear Signaling

Volume : 100 µL
Host : Mouse
Clonality : Monoclonal
Isotype : IgG1
GeneName : GATA5
GeneID : 140628
Uniprot : Q9BWX5
Specificity : GATA5 antibody detects endogenous levels of total GATA5.
Immunogen : Purified recombinant fragment of human GATA5 expressed in E. Coli.
Storage : Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human
Prediction :
Application : WB,ELISA
Dilution : ELISA 1 : 10000, WB 1 : 500-1 : 2000
Conj µgate : Unconj µgated
RRID : AB_2833948
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide :
Des# Blocking Peptide :
Blocking Peptide Price : $549 CAD

Volume : 100 µg
Purification : Immunogen affinity purified
Form : liquid
Purity : 95% as determined by SDS-PAGE
Host : Rabbit
Clonality : polyclonal Ab
Clone ID :
Isotype : IgG
Storage : PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.)
Background :
Immunogen : GATA binding protein 4
Aliases : GATA binding factor 4, GATA binding protein 4, GATA4, Transcription factor GATA 4
Observed MW : 45kd
Uniprot ID : P43694
Reactivity : Human, Mouse, Rat
Application : ELISA, WB, IHC, IP
Recommended dilution : WB : 1 : 500-1 : 1000; IP : 1 : 500-1 : 1000; IHC : 1 : 20-1 : 200
Gene ID : 2626
Research Area : Epigenetics, Signal Transduction, Metabolism, Cardiovasc µLar, Developmental biology, Stem Cells

Volume : 100 µg
Purification : Immunogen affinity purified
Form : liquid
Purity : 95% as determined by SDS-PAGE
Host : Rabbit
Clonality : polyclonal Ab
Clone ID :
Isotype : IgG
Storage : PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.)
Background :
Immunogen : GATA binding protein 4
Aliases : GATA binding factor 4, GATA binding protein 4, GATA4, Transcription factor GATA 4
Observed MW : 45-55kd
Uniprot ID : P43694
Reactivity : Human,Rat
Application : ELISA, WB
Recommended dilution : WB : 1 : 500-1 : 2000
Gene ID : 2626
Research Area : Epigenetics, Signal Transduction, Metabolism, Cardiovasc µLar, Developmental biology, Stem Cells

Volume : 50 µg
Clone Number :
Aliases : GATA3; Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3; GATA2; Endothelial transcription factor GATA-2; GATA-binding protein 2
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P23771/P23769
Immunogen : SyntheVolumed peptide derived from Human GATA-3 around the non-phosphorylation site of S308.
Raised in : Rabbit
Species Reactivity : Human, Mouse
Tested Applications : WB, IHC, IF, ELISA; IHC : 1 : 100-1 : 300, IF : 1 : 200-1 : 1000, ELISA : 1 : 5000
Background :
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conj µgate : Non-conj µgated
Buffer : Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATA3/GATA2
Research Areas : Epigenetics and Nuclear Signaling;Developmental biology;Immunology;Stem cells

Volume : 100 µg
Purification : Immunogen affinity purified
Form : liquid
Purity : 95% as determined by SDS-PAGE
Host : Rabbit
Clonality : polyclonal Ab
Clone ID :
Isotype : IgG
Storage : PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.)
Background : Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2(Th2) differentiation process following immune and inflammatory responses.
Immunogen : GATA binding protein 3
Aliases :
Observed MW : 45-50 kDa
Uniprot ID : P23771
Reactivity : Human, Mouse, Rat
Application : ELISA, WB, IHC
Recommended dilution : WB : 1 : 500-1 : 2000; IHC : 1 : 20-1 : 200
Gene ID : 2625
Research Area : Epigenetics, Metabolism, Cancer, Immunology, Developmental biology, Neuroscience

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : GATA2,GATA3
GeneID : 2624,2625
Uniprot : P23769/P23771
Specificity : GATA2/3 Antibody detects endogenous levels of total GATA2/3.
Immunogen : A syntheVolumed peptide derived from human GATA2/3, corresponding to a region within C-terminal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse,Rat
Prediction :
Application : WB,IHC,IF/ICC,ELISA(peptide)
Dilution : WB 1 : 1000-3000, IHC 1 : 200, IF/ICC, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2840911
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : DF2925-BP
Des# Blocking Peptide : GATA2/3 Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : GATA2
GeneID : 2624
Uniprot : P23769
Specificity : GATA2 Antibody detects endogenous levels of total GATA2.
Immunogen : A syntheVolumed peptide derived from human GATA2, corresponding to a region within the internal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse,Rat
Prediction : Bovine,Horse,Rabbit,Dog
Application : WB,IHC,IF/ICC,ELISA(peptide)
Dilution : WB 1 : 500-1 : 2000, IHC 1 : 50-1 : 200, IF/ICC 1 : 100-1 : 500, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2838084
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : DF6117-BP
Des# Blocking Peptide : GATA2 Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 50 µg
Clone Number :
Aliases : gata1-a antibody; gata1aGATA-binding factor 1-A antibody; Transcription factor xGATA-1A antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Xenopus laevis (African clawed frog)
UniProt ID : P23767
Immunogen : Recombinant Xenopus laevis GATA-binding factor 1-A protein (1-359AA)
Raised in : Rabbit
Species Reactivity : Xenopus laevis
Tested Applications : ELISA
Background : Transcription factor that acts synergistically with tal1/scl and lmo2 to specify embryonic dorsal mesoderm to a hematopoietic fate.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : gata1-a
Research Areas :

Volume : 50 µg
Clone Number :
Aliases : gata1-a antibody; gata1aGATA-binding factor 1-A antibody; Transcription factor xGATA-1A antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Xenopus laevis (African clawed frog)
UniProt ID : P23767
Immunogen : Recombinant Xenopus laevis GATA-binding factor 1-A protein (1-359AA)
Raised in : Rabbit
Species Reactivity : Xenopus laevis
Tested Applications :
Background : Transcription factor that acts synergistically with tal1/scl and lmo2 to specify embryonic dorsal mesoderm to a hematopoietic fate.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : gata1-a
Research Areas :

Volume : 50 µg
Clone Number :
Aliases : gata1-a antibody; gata1aGATA-binding factor 1-A antibody; Transcription factor xGATA-1A antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Xenopus laevis (African clawed frog)
UniProt ID : P23767
Immunogen : Recombinant Xenopus laevis GATA-binding factor 1-A protein (1-359AA)
Raised in : Rabbit
Species Reactivity : Xenopus laevis
Tested Applications : ELISA
Background : Transcription factor that acts synergistically with tal1/scl and lmo2 to specify embryonic dorsal mesoderm to a hematopoietic fate.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Biotin
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : gata1-a
Research Areas :

Volume : 50 µg
Clone Number :
Aliases : gata1-a antibody; gata1aGATA-binding factor 1-A antibody; Transcription factor xGATA-1A antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Xenopus laevis (African clawed frog)
UniProt ID : P23767
Immunogen : Recombinant Xenopus laevis GATA-binding factor 1-A protein (1-359AA)
Raised in : Rabbit
Species Reactivity : Xenopus laevis
Tested Applications : ELISA
Background : Transcription factor that acts synergistically with tal1/scl and lmo2 to specify embryonic dorsal mesoderm to a hematopoietic fate.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Non-conj µgated
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : gata1-a
Research Areas :

Volume : 50 µg
Clone Number :
Aliases : Anemia; X-linked; without thrombocytopenia; included antibody; ERYF 1 antibody; Eryf1 antibody; Erythroid transcription factor antibody; Erythrold transcription factor 1 antibody; GATA 1 antibody; GATA binding factor 1 antibody; GATA binding protein 1 (globin transcription factor 1) antibody; GATA binding protein 1 antibody; GATA-1 antibody; GATA-binding factor 1 antibody; GATA1 antibody; GATA1_HUMAN antibody; GF 1 antibody; GF-1 antibody; GF1 antibody; Globin transcription factor 1 antibody; NF E1 antibody; NF E1 DNA binding protein antibody; NF-E1 DNA-binding protein antibody; NFE 1 antibody; NFE1 antibody; Nuclear factor erythroid 1 antibody; Transcription factor GATA1 antibody; XLANP antibody; XLTDA antibody; XLTT antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P15976
Immunogen : Recombinant Human Erythroid transcription factor protein (39-153AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5\'-[AT]GATA[AG]-3\' within reg µLatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed : 24245781).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATA1
Research Areas : Epigenetics and Nuclear Signaling; Developmental biology; Stem cells

Volume : 50 µg
Clone Number :
Aliases : Anemia; X-linked; without thrombocytopenia; included antibody; ERYF 1 antibody; Eryf1 antibody; Erythroid transcription factor antibody; Erythrold transcription factor 1 antibody; GATA 1 antibody; GATA binding factor 1 antibody; GATA binding protein 1 (globin transcription factor 1) antibody; GATA binding protein 1 antibody; GATA-1 antibody; GATA-binding factor 1 antibody; GATA1 antibody; GATA1_HUMAN antibody; GF 1 antibody; GF-1 antibody; GF1 antibody; Globin transcription factor 1 antibody; NF E1 antibody; NF E1 DNA binding protein antibody; NF-E1 DNA-binding protein antibody; NFE 1 antibody; NFE1 antibody; Nuclear factor erythroid 1 antibody; Transcription factor GATA1 antibody; XLANP antibody; XLTDA antibody; XLTT antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P15976
Immunogen : Recombinant Human Erythroid transcription factor protein (39-153AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background : Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5\'-[AT]GATA[AG]-3\' within reg µLatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed : 24245781).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATA1
Research Areas : Epigenetics and Nuclear Signaling; Developmental biology; Stem cells

Volume : 50 µg
Clone Number :
Aliases : Anemia; X-linked; without thrombocytopenia; included antibody; ERYF 1 antibody; Eryf1 antibody; Erythroid transcription factor antibody; Erythrold transcription factor 1 antibody; GATA 1 antibody; GATA binding factor 1 antibody; GATA binding protein 1 (globin transcription factor 1) antibody; GATA binding protein 1 antibody; GATA-1 antibody; GATA-binding factor 1 antibody; GATA1 antibody; GATA1_HUMAN antibody; GF 1 antibody; GF-1 antibody; GF1 antibody; Globin transcription factor 1 antibody; NF E1 antibody; NF E1 DNA binding protein antibody; NF-E1 DNA-binding protein antibody; NFE 1 antibody; NFE1 antibody; Nuclear factor erythroid 1 antibody; Transcription factor GATA1 antibody; XLANP antibody; XLTDA antibody; XLTT antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P15976
Immunogen : Recombinant Human Erythroid transcription factor protein (39-153AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5\'-[AT]GATA[AG]-3\' within reg µLatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed : 24245781).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Biotin
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GATA1
Research Areas : Epigenetics and Nuclear Signaling; Developmental biology; Stem cells

Volume : 100 µL
Host : Mouse
Clonality : Monoclonal
Isotype : IgG1
GeneName : GATA1
GeneID : 2623
Uniprot : P15976
Specificity : GATA1 antibody detects endogenous levels of total GATA1.
Immunogen : Purified recombinant fragment of human GATA1 expressed in E. Coli.
Storage : Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human
Prediction :
Application : WB,IHC,IF/ICC,ELISA
Dilution : ELISA 1 : 10000, WB 1 : 500-1 : 2000, IHC 1 : 200-1 : 1000, IF/ICC 1 : 200-1 : 1000
Conj µgate : Unconj µgated
RRID : AB_2833811
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide :
Des# Blocking Peptide :
Blocking Peptide Price : $549 CAD