VAHTS TGS DNA Library Prep Kit for Oxford Nanopore Technologies ONT
Volume : 24 Rxns.
Keywords : DNA library construction; third generation library construction; TGS library construction; Nanopore sequencing; ONT sequencing
VAHTS TGS DNA Library Prep Kit for ONT is a ligation-based library construction kit suitable for Nanopore sequencing platform. Combined with the Barcode Adapter, it can perform single-sample or m µLti-sample analysis of 100 ng - 1.5 μg gDNA and amplicons. Build a database. This kit uses a directed evolution mutant enzyme with high catalytic activity and a caref µLly optimized buffer. It can directly perform Barcode Ligation without purification after end repair/damage repair/A-tail addition, and the experimental process is convenient. All reagents provided in the kit have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction.
Features :
1) The operation process is simple : steps of end repair + damage repair + adding A tail are completed in one tube, no purification is required, and Barcode connection can be performed directly
2) The time required for library construction is shortened : the minimum time required for the entire process of database construction can be <2 hours, which saves ≥30 minutes compared with the conventional process.
3) Compatible with various species you need to work with : compatible with animals, plants, microorganisms and other species
4) Excellent data quality : high read rate of long fragments, high data output, and good data quality
Better Data output and Quality
Species
Metric
TS201
Supplier A
Human
Clean Total Reads
1910,636
1754,451
Clean Total bases
37,568,318,787
30,769,214,717
Clean Mean quality(Mean Q)
14.77
14.69
Plant
Clean Total Reads
3,398,731
3,494,821
Clean Total bases
32,590,018,744
31687,125,980
Clean Mean quality(Mean Q)
12.86
12.77
Bacteria
Clean Total Reads
223,825
287,405
Clean Total bases
1629,142,727
1791295,356
Clean Mean quality(Mean Q)
15.5
15.3
Better Longer Reads
Species
DNA Volume
TS201
Supplier A
Human
5 - 50 kb
63.63%
60.95%
50-100 kb
8.25%
6.57%
100-200 kb
0.13%
0.11%
Total
72.01%
67.63%
Plant
5 - 50 kb
39.63%
37.02%
50-100 kb
2.22%
2.10%
100-200 kb
0.02%
0.02%
Total
41.87%
39.14%
Kit composition
Component
TS201-01 (24 rxns)
End Prep & Repair Buffer
36 µL
End Prep & Repair Master Enzyme Mix
36 µL
Barcode Rapid Ligase Mix
96 µL
MP Adapter Fast Ligation Buffer
240 µL
MP Adapter Fast Ligase
120 µL