Volume : 50 µL
Clone Number :
Aliases : CENP A antibody; CENP-A antibody; cenpa antibody; CENPA_HUMAN antibody; Centromere autoantigen A antibody; Centromere protein A 17kDa antibody; Centromere protein A antibody; Histone H3 like centromeric protein A antibody; Histone H3-like centromeric protein A antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P49450
Immunogen : Peptide sequence around site of Ser (7) derived from Human Histone H3-like centromeric protein A
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA, IHC; Recommended dilution : IHC : 1 : 10-1 : 100
Background : Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes (PubMed : 7962047, PubMed : 9024683, PubMed : 11756469, PubMed : 14667408, PubMed : 15702419, PubMed : 15475964, PubMed : 15282608, PubMed : 17651496, PubMed : 19114591 PubMed : 27499292, PubMed : 20739937). Replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore (PubMed : 18072184). The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3 (PubMed : 27499292, PubMed : 26878239). May serve as an epigenetic mark that propagates centromere identity thro µgh replication and cell division (PubMed : 15475964, PubMed : 15282608, PubMed : 26878239, PubMed : 20739937, PubMed : 21478274). Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress thro µgh mitosis, chromosome segregation and cytokinesis (PubMed : 11756469, PubMed : 14667408, PubMed : 18072184, PubMed : 23818633, PubMed : 25556658, PubMed : 27499292).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antigen Affinity Purified
Conj µgate : Non-conj µgated
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : CENPA
Research Areas : Epigenetics and Nuclear Signaling