Species Reactivity : Horse (Equus caballus; Equine)
UniProt : Q867B1
Abbreviation : EPO
Alternative Names : EP; MGC138142; MVCD2; epoetin
Application : ELISA
Range : 31.25-2000 pg/mL
Sensitivity : 11.6 pg/mL
Intra-AssayCV : ?4.6%
Inter-AssayCV : ?8.6%
Recovery : 1.05
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate EPO in samples. An antibody specific for EPO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEPO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for EPO is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPO bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Erythropoietin is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow.Also called hematopoietin or hemopoietin, it is produced by the peritub µLar capillary endothelial cells in the kidney, and is the hormone that reg µLates red blood cell production.It also has other known biological functions. For example, erythropoietin plays an important role in the brain's response to neuronal injury.EPO is also involved in the wound healing process.When exogenous EPO is used as a performance-enhancing dr µg, it is classified as an erythropoiesis-stim µLating agent (ESA). Exogenous EPO can often be detected in blood, due to slight difference from the endogenous protein, for example in features of posttranslational modification.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).