Species Reactivity : Cat (Felis catus,Feline)
UniProt : P19101
Abbreviation : TNF/TNFA/TNFSF12
Alternative Names : DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein|TNF superfamily; member 2|TNF; macrophage-derived|TNF; monocyte-derived|cachectin|tumor necrosis factor alpha
Application : ELISA
Range : 7.8-500 pg/mL
Sensitivity : 3.1 pg/mL
Intra-AssayCV : ?6.3%
Inter-AssayCV : ?10.2%
Recovery : 1.1
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate TNF/TNFA/TNFSF12 in samples. An antibody specific for TNF/TNFA/TNFSF12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNF/TNFA/TNFSF12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for TNF/TNFA/TNFSF12 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF/TNFA/TNFSF12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : TNFa is syntheVolumed as a 26 kDa, type II transmembrane protein that is 233 amino acids in length. It contains a 30 amino acid (aa) cytoplasmic domain, a 26 aa transmembrane segment, and a 177 aa extracell µLar region. TNFa is assembled intracell µLarly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNFa (sTNF-?is released from the C-terminus of the transmembrane protein thro µgh the activity of TNFa-converting enzyme (TACE), a membrane -bound disintegrin metalloproteinase. Rat cells known to express TNF-?include B cells, colonic columnar epithelial cells, NK and CD3 CD56 hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4 and CD8 T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).