Species Reactivity : Human (Homo sapiens)
UniProt : P04637
Abbreviation : TP53
Alternative Names : FLJ92943; LFS1; TRP53; p53; p53 antigen|p53 transformation suppressor|p53 tumor suppressor|phosphoprotein p53|transformation-related protein 53
Application : ELISA
Range : 78-5000 pg/mL
Sensitivity : 27 pg/mL
Intra-AssayCV : ?5.1%
Inter-AssayCV : ?7.9%
Recovery : 1.01
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate TP53 in samples. An antibody specific for TP53 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTP53 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for TP53 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TP53 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : The p53 responds to diverse cell µLar stresses to reg µLate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is post µLated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).