Species Reactivity : Human (Homo sapiens)
UniProt : P08861
Abbreviation : ELA3B
Alternative Names : RP11-26H16.3; CBPP; E1; EL-1; ELA3B; cholesterol-binding pancreatic protease|elastase 1|elastase 3B; pancreatic|fecal elastase 1|pancreatic elastase 1|pancreatic endopeptidase E|protease E|proteinas
Application : ELISA
Range : 0.312-20 ng/mL
Sensitivity : 0.135 ng/mL
Intra-AssayCV : ?4.5%
Inter-AssayCV : ?8.6%
Recovery : 0.9
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate ELA3B in samples. An antibody specific for ELA3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyELA3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for ELA3B is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ELA3B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Humans have six elastase genes which encode the structurally similar proteins elastase 1 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1 and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays..
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).