Species Reactivity : Human (Homo sapiens)
UniProt : P19544
Abbreviation : WT1
Alternative Names : AWT1; GUD; WAGR; WIT-2; WT33; OTTHUMP00000037553
Application : ELISA
Range : 0.156-10 ng/mL
Sensitivity : 0.063 ng/mL
Intra-AssayCV : ?3.6%
Inter-AssayCV : ?7.7%
Recovery : 0.99
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate WT1 in samples. An antibody specific for WT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for WT1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Wilms tumor protein is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a subset of patients with Wilms' tumor, the gene's namesake. M µLtiple transcript variants, res µLting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-A µg (C µg) translation initiation site upstream of, and in-frame with the first A µg, leading to additional isoforms.WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally reg µLated.The WT1 protein has been found to bind a host of cell µLar factors, e.g. p53, a known tumor suppressor.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).